甲状腺球蛋白自身抗体对2种化学发光免疫学方法检测甲状腺球蛋白的影响  

Evaluation of interference of thyroglobulin antibodies on the measurement of thyroglobulin by two chemiluminescence immunoassay

作　　者：程歆琦[1] 金成[1] 韩松[1] 张葵[1] 刘怀成[1] 谢少伟[1] 胡莹莹[1] 吴勤勇[1] 卢双玉[1] 杨国华[1] 张殿喜[1] 邱玲[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,100730

出　　处：《中华检验医学杂志》2015年第10期701-704,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨甲状腺球蛋白自身抗体（TgAb）对2种化学发光免疫学方法检测甲状腺球蛋白（Tg）的干扰。方法回顾性收集北京协和医院2012年11月至2015年4月检测TgAb和Tg的全部患者及查体者信息共计199315例，分析TgAb和Tg水平的关系，并计算查体人群TgAb的阳性率。收集290例TgAb浓度水平不同的患者血清，加入Tg确证血清进行回收实验，使用Pearson相关性分析比较Tg确证血清回收率与TgAb浓度的关系。结果按TgAb浓度进行分组，无论是健康查体人群、其他疾病人群还是分化型甲状腺癌（DTC）患者，Tg浓度均随着TgAh浓度的升高而降低。查体人群TgAb阳性率为10．84％（8416／77634），女性高于男性，随年龄增长而逐渐增加。Roche和Beckman系统TgAb阴性血清的Tg确证血清平均回收率分别为107．28％（86．30％～117．60％）和107．94％（85．60％-124．10％），回收实验结果可接受。Roche和Beckman系统TgAb阳性血清的Tg确证血清平均回收率分别为88．59％（35．85％-141．53％）和95．77％（36．48％～131．78％），回收率〈80％的分别占全部TgAb阳性血清的12．63％（24／190）和13．68％（26／190）。Tg确证血清回收率与TgAb水平呈显著负相关，Roche和Beckman系统的相关系数分别为r=-0．239（P=0．001）和r=-0．251（P〈0．001）。结论TgAb阳性，尤其是高浓度TgAb明显干扰tg的测定，检测Tg的标本均应该同时检测TgAb以了解是否存在TgAb对tg测定的干扰，同时当TgAb阳性时应告知临床医师其对Tg测定的干扰，以防贻误诊断和治疗。Objective To investigate the interference of thyroglobulin antibodies （TgAb） on the measurement of thyroglobulin （Tg） by 2 chemiluminescence immunoassays （ CLIAs）. Methods Data of 199 315 individuals with determined TgAb and Tg, including physical checkup subjects, differentiated thyroid carcinoma （DTC） patients and patients with other diseases, were retrospectively collected in Peking Union Medical College Hospital from November 2012 to April 2015. The correlation between serum Tg level and serum TgAb concentration was analyzed and the positive rate of TgAb in physical checkup subjects was calculated. Furthermore, 290 serum samples with different TgAb concentration were applied in the recovery test by adding in confirmed serum Tg. The correlation between the recovery of confirmed serum Tg and TgAb concentrations was evaluated using Pearson correlation analysis. Results The serum Tg was all decreased with the elevated TgAb concentration in each group of subjects. The positive rate of TgAb was 10. 84% （8 416/77 634） in physical checkup subjects. It was higher in females than in males and was increased with age. Recovery test showed that the average recoveries of confirmed serum Tg in TgAb-negative serum were 107.28% （86.30% - 117.60% ） and 107.94% （85.60% - 124.10% ） respectively in Roche and Beckman systems. But in TgAb-positive serum samples, the average recoveries in Roche and Beckman systems were 88.59% （35.85% - 141.53% ） and 95. 77% （36.48% - 131. 78% ） respectively, and 12. 63% （24/190） and 13.68% （26/190） samples displayed a recovery less than 80% . The recovery rateof confirmed serum Tg showed a significantly negative correlation with elevated TgAb concentration, with r = -0.239 （P=0.001） in Roche and r= -0.251 （P〈0.001） in Beckman. Conclusions TgAb-positive serum, especially with high concentration of TgAb, significantly interfered the measurement of Tg. Thus, serum TgAb should be determined together with serum Tg to explore whether there was an inter

关 键 词：甲状腺球蛋白 自身抗体 发光 免疫测定 甲状腺肿瘤 

分 类 号：R736.1[医药卫生—肿瘤]