固定化核苷磷酸化酶的制备及其性质研究  

Preparation of Immobilized Nucleoside Phosphorylase and Research on Its Property

作　　者：黄炯威 刘宁[2] 劳伟明 周华润 刘桂祯 

机构地区：[1]开平牵牛生化制药有限公司,广东江门529339 [2]厦门大学生命科学院,福建厦门361000

出　　处：《中国药业》2015年第20期32-34,共3页China Pharmaceuticals

摘　　要：目的优化核苷磷酸化酶的固定化工艺,并对固定化酶的性质进行研究。方法通过正交试验优化核苷磷酸化酶(包括嘌呤和嘧啶核苷磷酸化酶)固定化条件,并进一步对固定化核苷磷酸化酶的最适p H、温度及其稳定性进行研究。结果固定化酶条件为p H=8.0,温度为25℃,蛋白载量为20 mg/g载体,固定化时间为6 h;固定化核苷磷酸化酶的最适p H=7.5,最适温度为40℃,固酶连续使用20批次酶活力无明显损失。结论固定化核苷磷酸化酶稳定可靠,适合应用于工业化生产。Objective To optimize the immobilization process of the nucleoside phosphorylase and research on its property. Methods The immobilization process of the nucleoside phosphorylase（including purine and pyrimidine nucleoside phosphorylase） was optimized by orthogonal test, and took further study of the finest pH, temperature and stability of immobilized nucleoside phosphorylase. Results The finest condition of immobilized enzyme was pH =8.0,25 ℃ ,20 mg/g protein loads and immobilize for 6 h;the finest condition for the immobilized nucleoside phosphorylase to catalyze was pH ： 7.5 and 40 ℃. There was no significant loss of activity of the enzyme after use for catalyzing 20 batches consecutively. Conclusion The immobilized nucleoside phosphorylase is stable and reliable,which is suit- able to application in industrial production.

关 键 词：核苷磷酸化酶 固定化 制备 

分 类 号：R977.6[医药卫生—药品]