Repetitive Magnetic Stimulation Promotes Neural Stem Cells Proliferation by Upregulating MiR-106b In Vitro  

作　　者：柳华 韩肖华 陈红 郑彩霞 杨翼 黄晓琳 

机构地区：[1]Department of Rehabilitation Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology [2]College of Health Science,Wuhan Institute of Physical Education

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2015年第5期766-772,共7页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.81071601,and No.81171858)

摘　　要：Neural stem cells（NSCs） proliferation can be influenced by repetitive transcranial magnetic stimulation（r TMS） in vivo via micro RNA-106b-25 cluster,but the underlying mechanisms are poorly understood. This study investigated the involvement of micro RNA-106b-25 cluster in the proliferation of NSCs after repetitive magnetic stimulation（r MS） in vitro. NSCs were stimulated by r MS（200/400/600/800/1000 pulses per day,with 10 Hz frequency and 50% maximum machine output） over a 3-day period. NSCs proliferation was detected by using ki-67 and Ed U staining. Ki-67,p21,p57,cyclin D1,cyclin E,cyclin A,cdk2,cdk4 proteins and mi R-106 b,mi R-93,mi R-25 m RNAs were detected by Western blotting and q RT-PCR,respectively. The results showed that r MS could promote NSCs proliferation in a dose-dependent manner. The proportions of ki-67＋ and Edu＋ cells in 1000 pulses group were 20.65% and 4.00%,respectively,significantly higher than those in control group（9.25%,2.05%）. The expression levels of mi R-106 b and mi R-93 were significantly upregulated in 600–1000 pulses groups compared with control group（P〈0.05 or 0.01 for all）. The expression levels of p21 protein were decreased significantly in 800/1000 pulses groups,and those of cyclin D1,cyclin A,cyclin E,cdk2 and cdk4 were obviously increased after r MS as compared with control group（P〈0.05 or 0.01 for all）. In conclusion,our findings suggested that r MS enhances the NSCs proliferation in vitro in a dose-dependent manner and mi R-106b/p21/cdks/cyclins pathway was involved in the process.Neural stem cells（NSCs） proliferation can be influenced by repetitive transcranial magnetic stimulation（r TMS） in vivo via micro RNA-106b-25 cluster,but the underlying mechanisms are poorly understood. This study investigated the involvement of micro RNA-106b-25 cluster in the proliferation of NSCs after repetitive magnetic stimulation（r MS） in vitro. NSCs were stimulated by r MS（200/400/600/800/1000 pulses per day,with 10 Hz frequency and 50% maximum machine output） over a 3-day period. NSCs proliferation was detected by using ki-67 and Ed U staining. Ki-67,p21,p57,cyclin D1,cyclin E,cyclin A,cdk2,cdk4 proteins and mi R-106 b,mi R-93,mi R-25 m RNAs were detected by Western blotting and q RT-PCR,respectively. The results showed that r MS could promote NSCs proliferation in a dose-dependent manner. The proportions of ki-67＋ and Edu＋ cells in 1000 pulses group were 20.65% and 4.00%,respectively,significantly higher than those in control group（9.25%,2.05%）. The expression levels of mi R-106 b and mi R-93 were significantly upregulated in 600–1000 pulses groups compared with control group（P〈0.05 or 0.01 for all）. The expression levels of p21 protein were decreased significantly in 800/1000 pulses groups,and those of cyclin D1,cyclin A,cyclin E,cdk2 and cdk4 were obviously increased after r MS as compared with control group（P〈0.05 or 0.01 for all）. In conclusion,our findings suggested that r MS enhances the NSCs proliferation in vitro in a dose-dependent manner and mi R-106b/p21/cdks/cyclins pathway was involved in the process.

关 键 词：stimulation understood repetitive transcranial poorly manner staining involvement underlying Santa 

分 类 号：R741[医药卫生—神经病学与精神病学]