高效液相色谱-串联质谱法同时测定大鼠尿液中五种可能的癌症标志物  

Simultaneous Determination of Five Potential Cancer Biomarkers in Mouse Urine by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：郭岚[1] 孙玄[1] 鄢爱平[1] 柳英霞[1] 肖石妹 万益群[1] 

机构地区：[1]南昌大学化学学院,江西南昌330047

出　　处：《分析科学学报》2015年第5期611-615,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21105043;21465017);江西省自然科学基金(No.20122BAB203013)

摘　　要：建立了大鼠尿液中黄蝶呤、异黄蝶呤、蝶呤-6-羧酸、酪氨酸和色氨酸5种可能的癌症标志物的高效液相色谱-串联质谱（HPLC-MS/MS）分析方法。大鼠尿液除去蛋白后,稀释50倍直接进样分析,采用基质标准曲线法消除基质的影响。上述5种物质的仪器检出限分别为1.6、1.5、2.2、0.9和1.0ng/mL,加标回收率在89.0%~107.0%之间,相对标准偏差（RSD）在0.30%~5.19%之间。A simple,rapid and sensitive method for the simultaneous determination of five potential cancer biomarkers（xanthopterin,isoxanthopterin,pterin-6-carboxylic acid,tyrosine and tryptophan）in mouse urine by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）has been proposed.After the protein was removed,the mouse urine samples were diluted 50 times and then detected by HPLC-MS/MS directly.The influence of the matrix was eliminated by using the matrix standard curve.The limits of the instrument detection of the five analytes were 1.6,1.5,2.2,0.9and 1.0ng/mL,respectively.The recoveries were in the range of 89.0%-107.0%,and the relative standard deviations between 0.30%and 5.19%.

关 键 词：高效液相色谱-串联质谱法 大鼠尿液 癌症标志物 蝶呤类化合物 氨基酸 

分 类 号：O657.63[理学—分析化学]