红景天中红景天苷、酪醇、大花红天素及没食子酸提取工艺优选  

Optimization of extraction technology for salidroside,tyrosol,crenulatin and gallic acid in Rhodiolae Crenulatae Radix et Rhizoma with orthogonal test

作　　者：罗鑫[1,2] 王雪晶[1,2] 赵祎武[1,2] 黄文哲[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国中药杂志》2015年第18期3590-3593,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：优选大花红景天中红景天苷、酪醇、大花红天素及没食子酸的提取工艺。采用正交试验,以红景天苷、酪醇、大花红天素及没食子酸4种化合物的提取率为指标,选取乙醇体积分数、料液比、提取时间及提取次数为考察因素,优选4种化合物的最佳提取工艺。结果表明,以药材质量8倍量的70%乙醇回流提取2次,每次提取3 h为最佳提取工艺。该工艺所得红景天苷、酪醇、大花红天素及没食子酸的提取率均较高。优选的提取工艺稳定可行,4种化合物的收率高,可用于工业生产的放大。The extracting technology of salidroside,tyrosol,crenulatin and gallic acid from Rhodiolae Crenulatae Radix et Rhizoma was optimized. With extraction rate of salidroside,tyrosol,crenulatin and gallic acid as indexes,orthogonal test was used to evaluate effect of 4 factors on extracting technology,including concentration of solvent,the dosage of solvent,duration of extraction,and frequency of extraction. The results showed that,the best extracting technology was to extract in 70% alcohol with 8 times the weight of herbal medicine for 2 times,with 3 hours once. High extraction rate of salidroside,tyrosol,crenulatin and gallic acid were obtained with the present technology. The extracting technology was stable and feasible with high extraction rate of four compounds from Rhodiolae Crenulatae Radix et Rhizoma,it was suitable for industrial production.

关 键 词：大花红景天 红景天苷 酪醇 大花红天素 没食子酸 提取工艺 

分 类 号：R284.2[医药卫生—中药学]