猪源沙门氏菌携带I类整合子向人源细菌体外转移的研究  

Research on Class Ⅰ Integron Transmitting to Human Bacteria in Swine Salmonella in Vitro

作　　者：王学君[1] 卢春洪[1] 王源[1] 谭艾娟[1] 吕世明[2] 蒋庆庆[1] 符东顺 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学动物科学学院,贵州贵阳550025

出　　处：《天津农业科学》2015年第10期16-18,共3页Tianjin Agricultural Sciences

基　　金：贵阳市农业局科学技术计划项目筑科合(2008第5-1号);贵州大学"大学生创新创业训练计划项目"(201410657040)

摘　　要：为考察猪源沙门氏菌携带的I类整合子向人源细菌在体外接合试验中的转移频率,在体外接合试验中应用PCR方法检测细菌间I类整合子的转移。结果表明：人源沙门氏菌I类整合子转移效率在1.6×10-3~2.5×10-4 cfu·m L-1（供体菌）之间,人源大肠杆菌I类整合子转移效率在1.5×10-5~8.3×10-6 cfu·m L-1（供体菌）之间,猪源沙门氏菌I类整合子未转移给人源金黄色葡萄球菌。因此,猪源沙门氏菌I类整合子携带耐药基因盒在体外可向人源革兰氏阴性菌发生转移,且转移频率较高。To investigate the transfer frequency of class I integrons from swine Salmonella to human bacteria on the conjugation test in vitro, the transfertation of class I integrons between the strains was determined with PCR method. Results showed that the transfertation rate of class I integrons from swine Salmonella to human Escherichia coli were in 1.6 ×10-3~2.5 ×10-4cfu·m L-1（donor strain）,and to human Salmonella were 1.5 ×10-5--8.3 ×10-6cfu·m L-1（donor strain）. Class I integrons were no found in human in human Staphylococcus aureus. So, class I integrons of swine Salmonellae could transfer to gram-negative bacteria of human origin in vitro and the transfer frequency was quite high.

关 键 词：沙门氏菌 体外接合试验 整合子 转移 

分 类 号：R446.5[医药卫生—诊断学]