Foxp3^+ Tregs和PD1在胃癌组织中的表达及其临床病理意义  

Expression of Foxp3^+ Tregs and PD1 in gastric cancer tissues and their correlation with clinicopathological factors and prognosis

作　　者：侯婧瑛[1] 向仍运[2] 陈淑芬[3] 于钟[4] 吴淑云[4] 王林[5] 王凌云[4] 

机构地区：[1]中山大学附属孙逸仙纪念医院急诊科,广东广州510120 [2]湘西自治州人民医院消化内科,湖南吉首416000 [3]中山大学附属孙逸仙纪念医院重症医学科,广东广州510120 [4]中山大学附属孙逸仙纪念医院消化内科,广东广州510120 [5]中山大学附属孙逸仙纪念医院病理科,广东广州510120

出　　处：《中国病理生理杂志》2015年第10期1744-1749,共6页Chinese Journal of Pathophysiology

基　　金：广东省科技社会发展项目(No.2012B031800362)

摘　　要：目的:探讨胃癌组织中Foxp3+调节性T细胞(Foxp3+Tregs)与程序性死亡受体1(PD1)的表达情况及两者与胃癌患者临床病理和预后的关系。方法:采用免疫组织化学方法检测111例胃癌患者癌组织和20例正常胃黏膜组织中Foxp3+Tregs及PD1的表达情况,分析胃癌组织中两者的表达与患者临床病理和预后的关系及两项指标之间的相关性。结果:胃癌组织中Foxp3+Tregs和PD1表达增加,PD1表达于肿瘤浸润性淋巴细胞(TILs),两者的表达均与淋巴结转移及TNM分期相关(P<0.05),而与其它临床病理因素如肿瘤大小、病理类型,病变部位均无关,以上指标表达阳性者总体生存率低(P<0.05),预后差。胃癌组织中Foxp3+Tregs与PD1+TILs的表达之间存在显著的正相关(P<0.01)。结论:胃癌组织中存在Foxp3+Tregs和PD1+TILs共同表达,二者可作为判断胃癌患者疾病进展及预后的生物学标志物。AIM： To investigate the expression of Foxp3 ＋ regulatory T cells （ Foxp3 ＋ Tregs） and programmed death receptor 1 （ PD1 ） in gastric cancer tissues and their association with clinicopathological factors and prognosis of the patients. The correlation between the 2 molecules was also analyzed at the same time. METHODS： The tumor sections from 111 gastric cancer patients were stained for Foxp3 and PD1 by the method of immunohistochemistry. The associations of the expression levels of these 2 molecules with clinicopathological factors involved in the disease progression and progno- sis were statistically analyzed. The relationship of their expression was detected. RESULTS ： Foxp3 ＋ Tregs and PD1 were expressed in the gastric cancer tissues, and PD1 was expressed in the tumor infiltrating lymphocytes （TILs）. The expres- sion of Foxp3 and PD1 was correlated with lymph node metastasis, clinicopathological stage and prognosis of gastric cancer patients. The expression of these 2 determinants in the patients with lymph node metastasis and an advanced clinicopatho- logical stage was distinctly higher （P 〈 0. 05 ）. The patients with positive expression of the 2 indexes presented a lower overall survival rate and worse prognosis （P 〈 0. 05）. A significantly positive correlation between the infiltration of Foxp3 ＋ Tregs and the expression of PD1 ＋ TILs was also observed （P 〈0. 01 ）. CONCLUSION： Foxp3 ＋ Tregs and PD1 ＋TILs co- infiltrate in the gastric cancer tissues, which can be used as biological markers to predict the disease progression and prog- nosis.

关 键 词：胃癌 Foxp3’调节性T细胞 程序性死亡受体1 肿瘤浸润性淋巴细胞 临床病理 预后 

分 类 号：R363[医药卫生—病理学] R730.23[医药卫生—基础医学]