Zn^(2+)、Ca^(2+)和Mn^(2+)对丙酮丁醇发酵的协同影响  

Synergic effect of Zn^(2+),Ca^(2+),Mn^(2+) on acetone-butanol-ethanol fermentation

作　　者：付友思 吴又多[1] 陈丽杰[1] 

机构地区：[1]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《化工进展》2015年第10期3719-3724,共6页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(21376044);国家高技术研究发展计划(2011AA02A208;2012AA021205)项目

摘　　要：在丙酮丁醇发酵中共同添加0.001g/L ZnSO4·7H2O和4.0g/L CaCO3时,丁醇及总溶剂产量达到14.41g/L和23.69g/L,发酵终点乙酸和丁酸浓度为2.33g/L和1.02g/L。当共同添加0.001g/L ZnSO4·7H2O、4.0g/L CaCO3和0.8g/L MnSO4·H2O时,丁醇的比生成速率为0.48g/(g·h),相对于共同添加Zn2+和Ca2+条件下的丁醇比生成速率0.23g/(g·h)提高了108.69%,而发酵终点乙酸和丁酸浓度分别为1.99g/L和0.54g/L,同比分别降低了14.59%和47.06%。Zn2+、Ca2+和Mn2+3种金属离子对丙酮丁醇发酵具有正向协同调控作用。In acetone-butanol-ethanol（ABE） fermentation,when both 0.001g/L ZnSO4·7H2O and 4.0g/L CaCO3 were supplemented to the fermentation medium,the butanol and ABE production increased to 14.41g/L and 23.69g/L,respectively,while acetate and butyrate production in turn raised to 2.33g/L and 1.02g/L at the end of the fermentation. When 0.001g/L Zn SO4·7H2O,4.0g/L CaCO3 and 0.8g/L MnSO4·H2O were all supplemented to the fermentation medium,the specific butanol production rate increased from 0.23g/（g·h） to 0.48g/（g·h）,which increased by 108.69% compared to the control under both Zn2＋ and Ca2＋ supplementation condition. Moreover the acetate and butyrate production decreased to 1.99g/L and 0.54g/L,which decreased by 14.59% and 47.06% compared to the control under both Zn2＋ and Ca2＋ supplementation condition. These three metal elements of Zn2＋,Ca2＋ and Mn2＋ show dramatic synergic effects on ABE fermentation.

关 键 词：丁醇发酵 丙酮丁醇梭菌 金属元素 协同效应 

分 类 号：TQ923[轻工技术与工程—发酵工程]