博尔纳病病毒通过磷蛋白调节miR-134影响人少突胶质瘤细胞的增殖研究  

Borna disease virus encoded phosphoprotein down-regulates the proliferation of human oligodendrocytes by regulating miR-134

作　　者：白顺杰 周婵娟[1] 王明菊[1] 谢鹏[1] 

机构地区：[1]重庆医科大学神经科学研究中心,重庆400016

出　　处：《重庆医科大学学报》2015年第9期1181-1185,共5页Journal of Chongqing Medical University

基　　金：国家重大科学研究计划资助项目(编号:2009CB918302)

摘　　要：目的:探讨博尔纳病病毒(Borna disease virus,BDV)在感染宿主细胞中,miR-134对人少突胶质瘤细胞(oligodendrocyte,OL)增殖水平的影响。方法:应用real-time PCR方法检测正常的OL细胞和BDV感染的OL细胞(OL/BDV)中的miR-134表达水平,同时分别将BDV phosphoprotein(P24),nucleoprotein(P40)蛋白真核表达载体转染OL细胞,检测对miR-134的作用;通过miR-134过表达和BDV感染OL细胞,采用流式细胞仪检测miR-134和BDV对OL细胞增殖的影响。结果:real-time PCR表明miR-134在OL、OL/BDV细胞中的表达出现明显差异(P=0.000),并且BDV中的P24蛋白对miR-134有明显的上调作用(P=0.000),但P40对miR-134没有明显作用(P=0.139);流式细胞仪结果显示miR-134过表达(P=0.016)和BDV感染(P=0.001)均可以抑制OL细胞的增殖能力。结论:BDV感染可通过P24蛋白上调OL细胞中miR-134的表达,进而导致细胞增殖水平下降。Objective：To investigate the proliferation of oligodendrocyte（OL） cells induced by miR-134 in Borna disease virus（BDV） infection. Methods：The expression levels of miR-13d in OL and OL/BDV were detected by real-time PCR. BDV phosphoprotein （P24） or nucleoprotein（P40） gene expression vector was used to transfect OL cells respectively;the effects of BDV P24 and P40 pro- teins on the expression of miR-134 were detected by real-time PCR. The OL cells proliferation ability was investigated by flow cytom- etry（FCM）, along with miR-134 transfection and BDV infection. Results：There were significant differences in expression of miR-134 between OL and OL/BDV cells by real-time RT-PCR（P=0.000）. The expression of miR-134 was significantly increased by BDV P24 overexpression（P=0.000） ,but not by BDV P40（P=0.139）. Both overexpression of miR-134（P=0.016） and BDV infection（P=0.001） down-regulated the proliferation of OL cells. Conclusion ： BDV P24 protein down-regulates the proliferation of OL cells by up-regu- lating miR-134.

关 键 词：博尔纳病病毒 少突胶质瘤细胞 miR-134 P24 增殖 

分 类 号：R741.02[医药卫生—神经病学与精神病学]