粒状头样2在结直肠癌组织中的表达及意义  

Expression and clinical significance of GRHL2 in colorectal cancer

作　　者：全应军[1] 徐明 崔鹏[1] 叶敏[1] 庄彪[1] 闵志均[1] 

机构地区：[1]复旦大学附属浦东医院胃肠外科,上海201399

出　　处：《中华肿瘤杂志》2015年第10期764-768,共5页Chinese Journal of Oncology

摘　　要：目的：探讨结直肠癌组织中粒状头样2（ GRHL2）的表达情况及其与结直肠癌临床病理特征的关系。方法收集75例结直肠癌患者肿瘤组织及癌旁黏膜组织。采用免疫组化检测GRHL2的表达，采用实时荧光定量PCR（ qRT-PCR）和Western blot检测75例结直肠癌组织及其癌旁黏膜组织中的GRHL2基因和蛋白的表达水平。结果免疫组化结果显示，GRHL2蛋白主要表达于结直肠癌细胞核中，在结直肠癌组织中的阳性率（61．3％）高于癌旁黏膜组织（44．0％，P＜0．01）。 qRT-PCR结果显示，结直肠癌组织中GRHL2 mRNA的相对表达水平为（2．64±0．35），高于癌旁黏膜组织（1．19±0．23，P ＜0．001）；Ⅲ～Ⅳ期患者肿瘤组织中GRHL2 mRNA的表达水平（2．84±0．36）高于Ⅰ～Ⅱ期患者（2．31±0．32，P＜0．05）。 Western blot检测结果显示，GRHL2蛋白在结直肠癌组织中的表达水平高于癌旁黏膜组织（ P＜0．05）。 GRHL2的表达与结直肠癌患者的肿瘤大小、TNM分期和Ki-67指数均有关（均P＜0．05）。结论 GRHL2在结直肠癌组织中呈高表达，GRHL2的表达与结肠癌的临床病理特征有关。Objective The purpose of this study was to detect the expression of GRHL2 in colorectal cancer （ CRC ） tissues, and to assess the relationship between GRHL2 expression and clinicopathological features. Methods Immunohistochemistry was used to examine GRHL2 in 75 CRC tissues. GRHL2 mRNA and protein levels in the CRC tissues were also analyzed by qRT-PCR and Western blot. The relationship between GRHL2 and clinicopathological features was assessed by Pearson′s chi-square （χ2 ） test. Results Positive immunoreactivity for GRHL2 was detected in the nuclei of CRC cells. GRHL2 expression was increased in CRC tissues compared withthat in the paired non-tumor tissues （ 61. 3% vs. 44.0%, P〈0.01） . Moreover, qRT-PCR results showed that the relative expression level of GRHL2 mRNA in the colorectal cancer tissue was （2.64±0.35）, significantly higher than that of normal mucosa tissue （1.19± 0.23, P〈0.001）. The expression level of GRHL2 mRNA was higher in stage Ⅲ-Ⅳ patients（2.84±0.36） than that of stage Ⅰ-Ⅱ cases（2.31±0.32, P〈0.05）. Western blot results also showed that the expression level of GRHL2 protein in the colorectal carcinoma tissue was significantly higher than that in the normal mucosa （ P〈0.05） . GRHL2 expression was positively correlated with tumor size, TNM stage and Ki-67（ P〈0.05, respectively） . Conclusion Taking together, our findings demonstrate that GRHL2 is overexpressed in CRC, and plays an important role in the progression of CRC.

关 键 词：结直肠肿瘤 免疫组织化学 GRHL2 

分 类 号：R735.34[医药卫生—肿瘤]