珠江流域野生黄颡鱼Pelteobagrus fulvidraco的Cyt b基因序列分析  

Genetic Diversity of Mitochondrial Cyt b Sequences in Pelteobagrus fulvidraco from the Pearl River

作　　者：张鹤千 杨子拓 李桂峰[2] 肖诗斌[1,3] 孙际佳[1] 杨慧荣[1] 赵会宏[1] 谢堂晖 韩兴鹏[1,3] 刘丽[1,3] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]中山大学生命科学大学院,广东广州510275 [3]华南农业大学动物科学学院∥广东省农业动物基因组学与分子育种重点实验室,广东广州510642

出　　处：《中山大学学报（自然科学版）》2015年第5期102-108,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：公益性行业(农业)科研专项经费资助项目(201303048);国家科技基础条件平台建设资助项目(2008DKA30470-010)

摘　　要：利用PCR技术扩增得到珠江水系5个江段共190尾野生黄颡鱼Pelteobagrus fulvidraco样品的线粒体DNA细胞色素b(Cyt b)基因片段,并测定其序列,确定野生黄颡鱼的Cyt b基因片段全长为1 109 bp。在190尾样品中检测到43种不同的单倍型,单倍型多样性(Hd)和核苷酸多样性(Pi)分别为0.848 57和0.04 817,呈高单倍型、低核苷酸多样性的分布模式,表明其遗传多样性属于中等水平。利用Clustal-X 1.81和MEGA 5.0软件对190尾样品进行同源基因序列分析,其A+T含量(55.1%)显著高于G+C含量(44.8%),碱基组成差异不大,分布比较平均。使用最大似然率法构建分子系统发生树,结果表明都匀江段与左江江段的遗传分化程度最大(Fst=0.414 30)。AMOVA分析显示,珠江水系野生黄颡鱼的遗传变异均来自群体内,达到75.23%,表明地理因素对黄颡鱼的遗传分化产生了一定的影响,聚类结果与地理分布存在一定的相关性。中性检验结果表明,各群体间没有发生过种群扩张,种群数量相对稳定。The mitochondrial DNA cytochrome b（ Cyt b） gene segments of 190 yellow catfishes（ Pelteobagrus fulvidraco） from five river sections of Pearl river were amplified by PCR technique and sequenced. The complete sequence of the mitochondrial（ mt） Cyt b gene of Pelteobagrus fulvidraco was 1,109-bp in length. Sequence analysis indicated that there are 43 haplotypes in 190 individuals. The global haplotype diversity（ Hd） and nucleotide diversity（ Pi） was 0. 848 57 and 0. 048 17,respectively,indicating a pattern of high haplotype with low nucleotide diversity in the whole population,which indicated that the genetic diversity was a medium level. Sequence alignment performed by Clustal-X 1. 81 and MEGA 5. 0 softwares showed that AT content（ 55. 1%） was significantly higher than GC content（ 44. 8%） in these samples,and the base distribution was uniform. The genetic differentiation between Duyun and Zuojiang populations was the largest（ Fst= 0. 414 30） based on the molecular phylogenetic tree constructed by maximum likelihood method. The analysis of molecular variance（ AMOVA） showed that the genetic variation was 75. 23% merely presenting within populations rather than between populations,implying that geographical factors caused certain effects on genetic differentiation of Pelteobagrus fulvidraco. Results of neutral test showed that expansion was not happened among these five populations,therefore their population structures and quantities were relatively stable.

关 键 词：黄颡鱼 珠江流域 遗传多样性 CYT b 

分 类 号：Q953.5[生物学—动物学]