Toll样受体2基因多态性与胃癌及EB病毒相关胃癌易感性的关系  

TLR2 gene polymorphism and susceptibility to gastric carcinoma and EBV-associated gastric carcinoma

作　　者：卫美蓉 刘颂[1] 赵真真[1] 王笑峰[1] 罗兵[1] 

机构地区：[1]青岛大学医学院微生物学教研室,山东青岛266021

出　　处：《癌变．畸变．突变》2015年第5期341-346,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(30970157);山东省自然科学基金(ZR2011CM 016)

摘　　要：目的：探讨Toll样受体2（TLR2）r s3804099和rs3804100基因多态性与胃癌和EB病毒相关胃癌（EBVa GC）易感性的关系。方法：选用185例EBV阴性胃癌（EBVn GC）组织、41例EBVa GC组织以及100位健康人群外周血标本作为研究对象,采用PCR结合限制性片段长度多态性（RFLP）技术检测TLR2 r s3804099与rs3804100的基因多态性。结果：TLR2 r s3804099基因型和等位基因频率在胃癌组与健康对照组2 2间的差异均有统计学意义（χ=5.617,P=0.018;χ=6.467,P=0.011）,胃癌组C等位基因频率及C等位基因携带者频率均明显高于健康对2 2照组（χ=6.467,P=0.011;χ=4.444,P=0.035）,且与野生TT型相比,CC基因型可增加胃癌的发病风险（OR=3.554,95%CI=1.179~10.715）。TLR2 rs3804100位点的各基因型频率、C等位基因及C等位基因携带者的频率在胃癌组和健康对照组之间差异无统计学意义（P〉0.05）。TLR2 r s3804099与rs3804100位点的各基因型频率、C等位基因频率及C等位基因携带者频率在EBVa GC和EBVn GC两组间的差异均无统计学意义（P〉0.05）。结论：TLR2 rs3804099基因多态性可能与胃癌发病风险有关,C等位基因可能为胃癌的危险因子,携带C等位基因可能增加胃癌的发病风险。TLR2 r s3804099与rs3804100基因的多态性与EBVa GC的易感性无明显相关性。OBJECTIVE： The aim of our study was to evaluate the potential associations between the single nucleotide polymorphisms（SNPs） of Toll-like receptor（TLR2） rs3804099 and rs3804100 genes and the risk of gastric carcinoma（GC）,especially to Epstein-Barr virus-associated gastirc carcinoma（EBVa GC）. METHODS：TLR2rs3804099 and rs3804100 gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） in 185 cases of EBV-negative GC,41 cases of EBVa GC. 100 cases of peripheral blood samples from healthy individuals were also examined. The data was analysed by SPSS. RESULTS：As for the TLR2 gene（rs3804099）, there was significant difference between the GC group and the control group in both genotype and allelic 2 2frequencies（χ =5.617,P=0.018;χ =6.467,P=0.011）. The C allele and C allele carriers frequencies of gastric carcinoma 2 2group were significantly higher than those of controls（χ =6.467, P=0.011;χ =4.444,P=0.035）. Compared with the wild type TT, the CC genotype could increase the risk of gastric cancer（OR=3.554,95%CI=1.179-10.715）. As for the TLR2gene（rs38040100）,there were no significant differences between the GC group and the control group in genotype,C allelic frequency and C allele carrier frequency（P0.05）. In all the indicators,no polymorphism was found to be related to EBVa GC in the studied population（P0.05）. CONCLUSION：There was an association between TLR2 rs3804099 gene polymorphism and gastric carcinoma,and the C allele may be a risk factor for gastric carcinoma. Carrying the C allele may increase the risk of gastric carcinoma. No association was observed between TLR2（rs3804099 and rs38040100） gene polymorphism and EBVa GC.

关 键 词：胃癌 EB病毒 TOLL样受体 单核苷酸多态性 

分 类 号：R735.2[医药卫生—肿瘤]