K4在口腔黏膜下纤维性变早、中、晚期组织中的表达  

The expression of K4 in the tissue of oral submucous fibrosis at the early, middle and advanced stages

作　　者：魏高星[1] 吴颖芳[1] 谭爱红[1] 刘雄伟[1] 赵慧娟[1] 刘尔莎 

机构地区：[1]中南大学湘雅医院口腔医学中心,长沙410008

出　　处：《中国医师杂志》2015年第9期1296-1299,共4页Journal of Chinese Physician

摘　　要：目的 检测角蛋白（K）-4在口腔黏膜下纤维性变（OSF or OSMF）早、中、晚期组织中的表达,初步探讨K4在OSF发生、发展过程中的作用机制,为OSF的治疗提供新的靶点,为其疗效判断提供新的有效监测物.方法 收集10例正常颊黏膜组织;30例未经任何治疗的OSF患者颊黏膜组织,其中早期、中期、晚期各10例.运用免疫组化和Western blot蛋白印迹方法检测上述组织中K4的表达.结果 免疫组化结果显示,K4的表达主要定位在细胞质,阳性细胞可见于上皮层全层,呈棕黄色颗粒.在正常颊黏膜中,K4呈强阳性表达,高于OSF早、中、晚期病变组,差异均有统计学意义（P＜0.05）;K4在OSF早、中、晚期表达呈递减趋势,晚期显著低于早期,差异有统计学意义（P＜0.05）.Western blot结果显示,在正常颊黏膜中K4的表达量高于OSF早、中、晚病变组,差异均具有统计学意义（P＜0.05）;在OSF早、中、晚期颊黏膜组织中,K4的表达呈递减趋势,但三组之间差异无统计学意义（P＞0.05）.结论 K4在OSF早、中、晚期组织中的表达降低,可能参与了OSF发生发展过程.Objective To investigate the expressions of cytokeratin/keratin 4 in the buccal tissue of oral submucous fibrosis at the early,middle and advanced stages,explore which role keratin4 （K4） plays in the process and development of oral submucous fibrosis （OSF）,and provide evidence for K4 being a promising biomarker to evaluate the development and prognosis of OSF.Methods Ten cases of normal tissues,and 10 cases of OSF tissues with typical early,middle and advanced stages,were selected,respectively.Detect the expression of K4 in the tissue mentioned above through immunohistochemistry and Westem blot.The data was analyzed by statistical means.Results The results of immunohistochemistry showed that K4 was mainly located in cytoplasm,and positive cells with brownish yellow granules were seen in whole epithelial layer of the normal mucosa.The expression of K4 was lower at all stages of OSF than that at the normal tissue with statistical significance （P 〈0.05）.With the aggravation of OSF,the expression of K4 was decreased,difference between early and advanced stage was found to be statistically significant.The results of Western blot also showed that the expression of K4 was lower than that of early,middle and advanced stages of OSF （P 〈 0.05）.With the aggravation of OSF,the expression of K4 was decreased,but the differences between them had no significance （P 〉 0.05）.Conclusions The expression of K4 in OSF tissue of early,middle and advanced stages were decreased compared to normal tissue,respectively.It suggests that K4 might play and important role in the initiation and development of OSF.

关 键 词：口腔黏膜下纤维化/代谢 角蛋白4/代谢 

分 类 号：R781.5[医药卫生—口腔医学]