HR-HPV病毒负荷量及Nestin表达与宫颈病变的关系  

The study of correlation between high-risk types HPV load and expression of nestin in different cervical lesions

作　　者：阳婷[1] 吴佳捷[1] 赵延华[1] 郭慧[1] 刘心怡[1] 高雨琪 

机构地区：[1]中南大学湘雅医院妇产科,长沙410008

出　　处：《中国医师杂志》2015年第9期1356-1359,共4页Journal of Chinese Physician

摘　　要：目的 探讨高危型人乳头瘤病毒（HR-HPV）负荷量及巢蛋白（Nestin）表达与宫颈病变的关系.方法 采用第二代杂交捕获法（HC-Ⅱ）及免疫组化法对60例在湘雅医院首次行宫颈癌筛查并宫颈活检的患者行HR-HPV病毒负荷量及Nestin表达的检测.结果 （1）lgHPV（HR-HPV病毒负荷量的对数值）在宫颈高级别病变组中高于低级别病变组（P＜0.05）,HR-HPV病毒负荷量与宫颈病变程度呈正相关（rs=0.269,P=0.037）;（2）A组宫颈组织中Nestin阳性表达强度低于B、C组（H =7.271、22.843,P＜0.01）,C组宫颈组织中Nestin阳性表达强度高于B组（H=7.270,P＜0.01）,Nestin表达与宫颈病变程度呈正相关（r =0.646,P=0.000）;（3）宫颈病变组织中HR-HPV病毒负荷量与Nestin表达呈正相关（P＜0.05）.结论 HR-HPV病毒负荷量及Nestin阳性表达与宫颈病变密切相关,两者的联合检测对预测宫颈病变及癌前病变的进展有一定的参考价值,对早期防治宫颈癌有指导意义.Objective To investigate the association between high-risk types of human papillomavirus （HR-HPV） load and the expression of nestin in different cervical lesions.Methods The hybridization capture Ⅱ （HC-Ⅱ） assay was used to test the HR-HPV load from 60 patients who were the first time to do the cervical cancer screening in Xiangya Hospital,and immunohistochemistry was used to detect the expression of nestin in those biopsy tissue samples.Results （1） The lgHPV level （logarithm of HR-HPV load） in the high level lesion group was higher than the low level one （P 〈0.05）,and HR-HPV load was positively associated with the degree of cervical lesions （rs =0.269,P =0.037）.（2） The expression of nestin in A group was weaker than groups B and C （H =7.271,22.843,P 〈0.01）,and the expression of nestin in C group was stronger than B group （H =7.270,P 〈0.01）,and the expression of nestin was positively associated with the degree of cervical lesion （rs =0.646,P =0.000）.（3） The HR-HPV load was positively associated with the expression of nestin （P 〈 0.05）.Conclusions The HR-HPV load and the expression of nestin are closely related to the cervical lesions,and the joint detection has a referential value for early prevention of cervical lesions and prediction of progress of cervical precancerous lesion.It might guide the early prevention and treatment of cervical cancers.

关 键 词：乳头状瘤病毒科/代谢 病毒载量 中间丝蛋白质类/代谢 神经组织蛋白质类/代谢 宫颈疾病/代谢 

分 类 号：R737.33[医药卫生—肿瘤]