大鼠缺血性卒中模型MRI与TTC染色法测量脑梗死体积的对比研究  

Comparative study of MRI and 2,3,5-triphenyltetrazolium chloride(TTC) staining for quantification rat brain infarct volume

作　　者：程晓青[1] 李建瑞[1] 方晓堃 李延军[1] 张薇[1] 张泳 卢光明[1] 

机构地区：[1]南京军区南京总医院医学影像科,江苏南京210002 [2]通用电气医疗集团,上海201203

出　　处：《医学影像学杂志》2015年第10期1871-1873,共3页Journal of Medical Imaging

基　　金：国家自然科学基金;青年科学基金项目(81201072);南京军区南京总医院内课题(2013054)

摘　　要：目的对比活体下MRI与大体标本2,3,5-氯化三苯基四氮唑(TTC)染色法评估缺血性卒中大鼠脑梗死体积的差异。方法对19只大鼠利用线栓法建立急性缺血性卒中模型,并于造模24h后行头颅MRI扫描,扫描完毕后进行TTC染色,利用配对t检验评价T2WI及TTC染色测量脑梗死体积的统计学差异。结果除2只大鼠死亡外,共有17只纳入本研究中。T2WI序列与TTC染色法测量的脑梗死体积无明显统计学差异(t=0.50,P=0.62)。结论对于有MRI条件的动物实验研究,T2WI序列可以取代TTC染色法快速、准确的测量缺血性卒中动物模型的脑梗死体积,简化实验流程。Objective To compare the difference between MRI in vivo and 2,3,5-triphenyltetrazolium chloride （TTC） staining for evaluation the rat＇s brain infarct volume. Methods 19 rats were subjected to forever middle cerebral artery occlusion. After 24 hours of the model-establishing, MRI was acquired to observe the infarct volume. After that, TTC staining was implemented. The pair t test was used to evaluate the statistics difference of brain infarct volume analyzed by TTC staining and T2WI. Results Except the death of 2 rats, 17 rats were included in this study. Measurement of brain infarction volume by T2 WI and TTC staining was no significant statistics difference （ t = 0.50, P = 0.62）. Conclusion For animal experiment research, T2 WI can rapidly and accurately replace TTC staining for measuring the animal brain infarct volume, and simplifying the experiment process.

关 键 词：磁共振成像 脑梗死 大鼠 氯化三苯基四氮唑染色 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]