乙酰葛根素对Aβ_(25-35)诱导BV-2小胶质细胞Caspase-3表达的影响  

Effect of acetylpuerarin on the expression of caspase-3 in BV-2 microglia induced by Aβ_(25-35)

作　　者：李梅[1] 孟庆慧[1] 蔡巧英[1] 徐雁[1] 范晓婷[1] 

机构地区：[1]潍坊医学院护理学院,山东潍坊261053

出　　处：《山东大学学报（医学版）》2015年第10期32-36,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(ZR2013HL069;ZR2010HM132);山东省医药卫生科技发展计划(2014WS0467);潍坊医学院科技创新研究基金(K1301017)

摘　　要：目的探究乙酰葛根素对β淀粉样蛋白(Aβ25-35)诱导BV-2小胶质细胞形态及含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)表达的影响。方法用凝聚态Aβ25-35诱导体外培养的BV-2小胶质细胞作为阿尔茨海默病炎症细胞模型,将细胞分为对照组、模型组、Caspase-3抑制剂(Z-DEVD-fmk)组以及乙酰葛根素低(0.1μmol/L)、中(0.4μmol/L)、高(1.6μmol/L)剂量共6组,采用倒置相差显微镜观察小胶质细胞形态变化,应用Western blotting检测Caspase-3蛋白表达水平。结果形态学结果显示,Aβ25-35可激活小胶质细胞使其由静息态变为阿米巴样,Caspase-3抑制剂、乙酰葛根素可改善Aβ25-35诱导的小胶质细胞形态改变。Western blotting结果显示,与对照组相比,模型组Caspase-3表达显著升高,差异有统计学意义(P<0.01);与模型组比较,Caspase-3抑制剂、乙酰葛根素各剂量组Caspase-3表达均显著降低(P<0.01);与Caspase-3抑制剂组相比,乙酰葛根素低、中剂量组Caspase-3表达均显著升高(P<0.01),高剂量组则无统计学意义。结论乙酰葛根素可抑制Aβ诱导BV-2小胶质细胞激活,其机制可能与降低Caspase-3表达有关,且以高剂量组效果较好。Objective To investigate the effect of acetylpuerarin on the expression of Caspase-3 in BV-2 microglia induced by Aβ25-35. Methods BV-2 microglia cells were activated with condensed Aβ25-35 as Alzheimers disease inflammatory cell model,which were then divided into 6 groups： control group,model group,Caspase-3 inhibitor（ Z-DEVDfmk） group and acetylpuerarin groups（ low-dose group 0. 1 μmol / L,moderate-dose group 0. 4 μmol / L,high-dose group 1. 6 μmol / L）. Morphological changes of microglia cells were observed with an inverted phase contrast microscope,and the expression level of Caspase-3 protein in each group was detected by Western blotting. Results It was observed that Aβ25-35 activated microglia from resting state into ameboid,and both Caspase-3 inhibitor and acetylpuerarin improved cell morphological changes induced by Aβ25-35. Western blotting results showed that compared with the control group,the model group had significantly increased expression of Caspase-3（ P〈0. 01）; compared with the model group,Caspase-3 inhibitor group and acetylpuerarin groups had markedly reduced expression of Caspase-3（ P〈0. 01）;compared with Caspase-3 inhibitor group,acetylpuerarin low-dose and moderate-dose group had significantly increased expression of Caspase-3（ P〈0. 01）,while acetylpuerarin high-dose group had no statistically significant difference.Conclusion Acetylpuerarin can inhibit activation of BV-2 microglia cells induced by Aβ25-35. The mechanism may be related to decreased expression of Caspase-3,especially high-dose acetylpuerarin.

关 键 词：阿尔茨海默病 乙酰葛根素 Β淀粉样蛋白 Caspase-3 WESTERN BLOTTING 小胶质细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]