香菇L808菌株自交子代在变温培养中菌丝生长速度的分化  

Effect of Exposure to Poikilothermic Regimes on Mycelial Growth Rate Distribution Patterns Exhibited by 164 Self-bred Dikaryons Derived from Lentinula edodes Parent Strain L808

作　　者：宋春艳[1] 杨晓春[1] 张丹[1] 奚莉萍 章炉军[1] 于海龙[1] 尚晓冬[1] 谭琦[1] 

机构地区：[1]上海市农业科学院食用菌研究所,上海市农业遗传育种重点开放实验室,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海201403

出　　处：《食用菌学报》2015年第3期17-20,2,共4页Acta Edulis Fungi

基　　金：沪农青字(2014)第1-16号;现代农业产业技术体系建设专项资金资助(CARS24);沪农科推字[(2013)第1-2号]的部分研究内容

摘　　要：以香菇（Lentinula edodes）L808菌株为亲本，用多孢自交方法取得164个自交子代菌株，连同亲本分别于填充木屑培养料的大试管中进行6个梯度的变温培养E25、30、32、34、25（恢复）、25℃（生长），每个温度培养5d]，考察变温处理下菌丝生长速度的变化和分化。结果表明，菌丝生长速度明显受到变温的影响，30～34℃的高温对所有菌株造成了不同程度的刺激，返回25℃后菌株的恢复和恢复后的生长等均呈现明显分化，供试群体中没有表现完全相同的个体，反应了菌丝生长速度这一性状背后复杂的基因调控体系；而且变温培养的菌丝生长速度应该和恒温培养的菌丝生长速度一样成为菌株特性评价的重要指标。A total of 164 self-bred dikaryotic progenies derived from Lentinula edodes strain L808 were selected from 207 isolates based on the formation of rnyclial clamp connections and, together with the parent stain, were inoculated in glass tubes filled with a substrate consisting of 80% sawdust and 20% wheat bran （moisture content 50%, pH 6.5）. After the mycelia had colonized the surface of the substrate, the tubes were subjected to six temperature regimes.. 25℃ ,30℃ ,32℃ ,34℃, 25 ℃ （recovery） and 25℃ （growth）, with each incubation period lasting five days. Mycelial growth rates （MGR） were recorded in each case. Our data revealed that MGR of the isolates varied considerably during exposure to the poikilothermic regimes and no isolate exhibited the same MGR throughout the entire experimental period, indicating a complex system of gene regulation. Consequently, the MGR under constant temperature and the MGR under poikilothermic regimes must both be taken into account in strain evaluation, especially when the mushroom cultivation period is long and the ambient temperatures during growth are highly variable.

关 键 词：香菇 菌丝生长速度 分化 变温处理 

分 类 号：S646.12[农业科学—蔬菜学]