七氟醚预处理对脊髓缺血再灌注大鼠5脂氧合酶表达的影响  

Effect of sevoflurane preconditioning on expression of 5-lipoxygenase during spinal cord ischemia-reperfusion in rats

作　　者：欧军[1] 赵红[2] 谢红[1] 姚平波[1] 苏小桃[1] 何军[1] 何俊[1] 

机构地区：[1]南华大学附属南华医院脊椎外科,湖南衡阳421002 [2]南华大学护理学院基础护理教研室,湖南衡阳421001

出　　处：《中国现代医学杂志》2015年第25期12-15,共4页China Journal of Modern Medicine

基　　金：湖南省自然科学基金资助项目(No:2013FJ4212);湖南省教育厅资助项目(No:14B157)

摘　　要：目的探讨七氟醚预处理对大鼠脊髓缺血再灌注损伤时5脂氧合酶（5-LOX）表达的影响。方法雄性成年SD大鼠45只,体重250~300 g,采用随机数字表法,将大鼠随机分为3组（n=15）：假手术组（S组）、脊髓缺血再灌注组（I/R组）和七氟醚预处理组（I组）。采用胸主动脉球囊阻断＋体循环低血压法制备大鼠脊髓缺血再灌注模型。I组吸入3.4%七氟醚2 h,24 h后制备模型。于再灌注24 h时采用神经功能缺陷评分（NDS）法评价大鼠神经功能,随后处死取脊髓,分别采用Western blot法和RT-PCR法测定5-LOX、p-ERK1/2和t-ERK1/2蛋白及其m RNA的表达水平。应用ELISA检测血清中肿瘤坏死因子-α（TNF-α）的含量。结果与S组比较,I/R组和I组神经功能缺陷评分升高,I/R组5-LOX和p-ERK1/2蛋白及其m RNA表达上调,TNF-α含量升高,I组5-LOX m RNA和p-ERK1/2蛋白及其m RNA表达上调（P〈0.05）;与I/R组比05）。结论七氟醚预处理可能通过下调5-LOX表达,抑制ERK1/2信号通路,从而减轻大鼠脊髓缺血再灌注损伤。【Objective】 To investigate the effect of sevoflurane preconditioning on the expression of 5-lipoxygenase（5-LOX） during spinal cord ischemia-reperfusion（I/R） in rats. 【Methods】 Forty-five male adult Sprague-Dawley rats weighing 250~300g were randomly divided into 3 groups（n = 15 each）： sham operation group（group S）; spinal cord I/R group（group I/R）; sevoflurane preconditioning group（group I）. The animals were anesthetized with intraperitoneal 4% chloral hydrate 0.1 mg/kg. Spinal cord ischemia was induced by 10 min occlusion of the thoracic aorta combined with controlled hypotension（MAP = 40 mm Hg）. The rats inhaled 3.4%sevoflurane for 2 h and spinal cord I/R was produced 24 h later in group I. The neurological deficits were scored at24 h of reperfusion. The animals were then sacrificed. The expression of 5-LOX, p-ERK1/2 and t-ERK1/2 protein and m RNA was detected using Western blot and RT-PCR respectively. TNF-α levels in serum were detected by ELISA. 【Results】 Compared with group S, the neurological deficit score and TNF-α levels in serum were significantly increased in groups I/R and I, the expression of 5-LOX and p-ERK1/2 protein and m RNA was up-regulated in group I/R, and the expression of 5-LOX m RNA and p-ERK1/2 protein and m RNA was up-regulated in group I（P〈0.05）. Compared with group I/R, the neurological deficit score was significantly lower, TNF-α levels in serum was lower, and the expression of 5-LOX and p-ERK1/2 protein and m RNA was lower in group I（P〈0.05）.【Conclusion】Sevoflurane preconditioning can reduce spinal cord I/R injury by down-regulating the expression of5-LOX and inhibiting ERK1/2 signaling pathway in rats.

关 键 词：生四烯酸盐5-脂氧合酶 七氟醚 缺血预处理 再灌注损伤 脊髓 

分 类 号：R-332[医药卫生]