经导管建立兔急性肾动脉血栓栓塞模型  

Establishment of rabbit model of acute renal artery thrombosis through catheter

作　　者：沈长银[1] 彭虹[1] 石蓓[1] 刘汉林[1] 刘志江[1] 赵然尊[1] 许官学[1] 

机构地区：[1]遵义医学院附属医院心内科,贵州遵义563003

出　　处：《中国现代医学杂志》2015年第25期69-72,共4页China Journal of Modern Medicine

基　　金：贵州省中医药管理局中医药;民族医药科学技术研究课题资助项目(No:QZYY2011-72)

摘　　要：目的 探讨导管法建立兔急性肾动脉血栓栓塞模型的可行性,为研究急性肾动脉血栓栓塞及急性肾功能衰竭提供可靠实验室方法学。方法 新西兰大白兔20只,随机分为栓塞组及对照组,每组10只。栓塞组经股动脉置管,自制血栓经导管行选择性兔肾动脉栓塞,记录栓塞前后主动脉压力变化情况,分别于术前,术后1、2及10 h抽血化验肾功能,然后切取兔肾脏后处死动物,标本行病理学检查;对照组只行股动脉置管,不行血栓栓塞,分别在相应时间点行肾功能及病理学检查。结果 栓塞组8只兔成功行单侧肾动脉栓塞,2只置管失败,肾动脉造影结果提示手术即刻肾动脉栓塞成功,主动脉压力在栓塞成功后比栓塞前升高（〈0.05）。栓塞术后2 h兔肾功能肌酐及尿素氮升高,术后10 h升高明显并同时有乳酸脱氢酶升高,对照组肾功能及乳酸脱氢酶无明显变化（〈0.05）。栓塞组在栓塞后10 h肾脏病理学证实血栓栓塞组肾脏可见肾皮质梗死约占肾皮质70%~90%,梗死灶周围肾脏组织充血、出血伴中性粒细胞浸润。对照组无肾梗死,仅见肾脏稍充血,肾小管轻微水肿（〈0.05）。结论 经导管建立兔急性肾动脉血栓栓塞模型成功率高,栓塞术后肾功能及病理学变化符合临床急性肾动脉血栓变化过程,此方法简单易行,可控制性好,能为急性肾动脉栓塞临床治疗及药物干预研究提供可靠动物模型。【Objective】To explore the feasibility of establishing the model of acute renal artery thrombosis in rabbits by catheter method,and provide reliable laboratory method for the study of acute renal artery thrombosis and acute renal failure. 【Methods】Twenty New Zealand white rabbits were randomly divided into embolization and control groups with ten in each group. The embolization group was performed with the femoral artery catheter, and the renal artery embolization was performed with self-made thrombus, then the changes of aortic pressure before and after operation were recorded, kidney function were tested before the operation, 1 hour, 2 hours and 10 hours after the operation. Then the rabbits were killed and kidneys were cut for pathological examination. While the control group was only received femoral artery catheterization. The renal function and pathological examination were underwent in the corresponding time. 【Results】Eight rabbits in the embolization group underwent unilateral renal artery embolization, and catheterization was failed in 2 rabbits. Renal artery angiography showed that the operation was successful. The aortic pressure raised after embolization（P〈0.05）. After 2 hours of embolization, serum creatinine and urea nitrogen of rabbits raised, they were significantly increased and the lactate dehydrogenase raised 10 hours after operation. The renal function and lactic dehydrogenase of control group had no obvious changes（P〈0.05）.Kidney pathological changes confirmed that the embolism renal cortical infarction occured in 70%-90% of renal cortex, kidney tissue hyperemia, hemorrhage and infiltration of neutrophils were found around the infarction focus.The control group had no renal infarction, slight hyperemia in kidney was seen, slight edema was found in renal tubules（P〈0.05）. 【Conclusions】 The establishment of rabbit model of acute renal artery thrombosis through catheter had a high success rate. Renal functions and pathological changes were similar with the clinica

关 键 词：肾动脉造影 兔 急性肾功能衰竭 肾动脉栓塞 

分 类 号：R-332[医药卫生]