Cupriavidus metallidurans SHE好氧还原Se(Ⅳ)合成硒纳米颗粒的特性研究  

Biosynthesis of selenium nanoparticles through aerobic selenite reduction by Cupriavidus metallidurans SHE

作　　者：沈娥[1] 刘紫嫣[1] 周豪[2] 李端行 张旭旺[1] 王经伟[1] 沈文丽[1] 曲媛媛[1] 周集体[1] 

机构地区：[1]工业生态与环境工程教育部重点实验室大连理工大学环境学院,大连116024 [2]工业生态与环境工程教育部重点实验室大连理工大学盘锦校区食品与环境学院,盘锦124000

出　　处：《环境科学学报》2015年第10期3065-3070,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.21176040);新世纪优秀人才支持计划(No.NCET-13-0077);中央高校基本科研业务费专项资金(No.DUT14YQ107)~~

摘　　要：利用贪铜杆菌(Cupriavidu smetallidurans)SHE在好氧条件下还原Se(Ⅳ)生成硒纳米颗粒,考察不同条件对还原过程的影响,并对还原产物进行表征.结果显示,菌株SHE还原Se(Ⅳ)的最适条件为pH=8、温度30℃、底物浓度1.0 mmol·L-1,在此条件下Se(Ⅳ)的还原率最高,可达100%.通过紫外光谱扫描、微观形貌分析、粒度分析及X射线衍射分析表明,合成的硒纳米颗粒为六方晶型,粒径为(130.2±27.0)nm.研究结果表明,菌株SHE可有效的还原Se(Ⅳ)生成硒纳米颗粒,为微生物合成纳米硒的潜在应用提供参考.Cupriavidus metallidurans strain SHE was used for the aerobic reduction of Se（ Ⅳ） to selenium nanoparticles（ Se NPs）. The effects of different reaction conditions on Se（ Ⅳ） reduction were investigated,and the reduction product was characterized as well. The results showed that the optimal conditions for Se（ Ⅳ） reduction by strain SHE were as follows： pH 8,temperature 30 ℃,and Se（ Ⅳ） 1. 0 mmol·L-1,which led to a completely reduction of Se（ Ⅳ）. Based on the analysis of UV-vis spectroscopy,microscopic morphology,size distribution analysis and X-ray diffraction,Se NPs were in hexagonal phase with a diameter of 130. 2 nm ± 27. 0 nm. This study indicated that strain SHE could be effectively exploited for Se NPs synthesis by Se（ Ⅳ） reduction,which would contribute to the potential application of biogenic Se NPs.

关 键 词：贪铜杆菌 亚硒酸盐 硒纳米颗粒 微生物合成 

分 类 号：X172[环境科学与工程—环境科学]