正交试验优化比色法测定马铃薯还原糖实验条件研究  

Optimization of process for determination of reducing sugar content in potato by 3,5-2 Nitrosalicylate colorimetric method

作　　者：童丹[1] 

机构地区：[1]定西师范高等专科学校,甘肃定西743000

出　　处：《中兽医医药杂志》2015年第5期43-44,共2页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省高等学校研究生导师项目(121901)

摘　　要：马铃薯薯片和薯条油炸色泽是评价马铃薯油炸食品质量优劣的重要指标,还原糖含量则是影响其油炸色泽的重要因素。测定马铃薯还原糖含量的方法较多,3,5-二硝基水杨酸(DNS)比色法是其中的一种常用方法,具有简便、快速、灵敏度高等特点。为确定3,5-二硝基水杨酸(DNS)比色法测定马铃薯还原糖含量的最佳实验条件,以本地主栽品种"新大坪"为试验材料,以正交试验优选其最佳测定条件。结果该方法的最佳条件为显色剂用量3 m L、显色时间5 min、前处理时间15 min时,测得的吸光度值最大为0.558。The color is an important index of evaluating the quality of potato fries, and reducing sugar content is a major factor which has effect on color of fried potatoes. There are many ways of determining reducing sugar content in potato, among which 3, 5-2 nitro salicylic acid（DNS） colorimetric method is simple, fast, high sensitive and commonly used. In order to optimize the process for determining reducing sugar content in potato by DNS colorimetric method, the main variety ＂Xin Daping＂was used as testing material, and orthogonal test was carried out to determine the optimum experimental conditions.The results showed that the absorbance value achieved maximum of 0.558 when the dosage of color-developing agent was 3m L, developing time was 5 min and the pre-processing time was 15 min.

关 键 词：马铃薯 还原糖 3 5-二硝基水杨酸(DNS)比色法 测定条件 

分 类 号：S532[农业科学—作物学]