Bienzyme system immobilized in biomimetic silica for application in antifouling coatings  

仿生固定化多酶及其在抗生物黏泥涂层中的应用（英文）

作　　者：王洪武 姜艳军 周丽亚 高静 

机构地区：[1]School of Chemical Engineering,Hebei University of Technology [2]National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences

出　　处：《Chinese Journal of Chemical Engineering》2015年第8期1384-1388,共5页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(21006020,21276060,21276062);the Application Basic Research Plan Key Basic Research Project of Hebei Province(11965150D);the Natural Science Foundation of Tianjin(13JCYBJC18500)

摘　　要：Antifouling coatings are used extensively on vessels and underwater structures. Conventional antifouling coatings contain toxic biocides and heavy metals, which may induce unwanted adverse effects such as toxicity to non-target organisms, imposex in gastropods and increased multiresistance among bacteria. Therefore,enzyme-based coatings could be a new alternative solution. A H2O2-producing bienzyme system was developed in this study. H2O2 can be produced from starch by the cooperation of α-amylase and glucose oxidase, which promotes the hydrolysis of polymeric chain and oxidizes the glucose to produce H2O2, respectively. The encapsulated bienzyme(A-G@BS) exhibits enhanced stabilities of thermal, pH, recycling and tolerance of xylene. The A-G@BScontaining coating releases H2O2 at rates exceeding a target of 36 nmol·cm-2·d-1for 90 days in a laboratory assay. The results demonstrate that the method is a promising coating technology for entrapping active enzymes,presenting an interesting avenue for enzyme-based antifouling solutions.Antifouling coatings are used extensively on vessels and underwater structures. Conventional antifouling coatings contain toxic biocides and heavy metals, which may induce unwanted adverse effects such as toxicity to non-target organisms, imposex in gastropods and increased multiresistance among bacteria. Therefore,enzyme-based coatings could be a new alternative solution. A H2O2-producing bienzyme system was developed in this study. H2O2 can be produced from starch by the cooperation of α-amylase and glucose oxidase, which promotes the hydrolysis of polymeric chain and oxidizes the glucose to produce H2O2, respectively. The encapsulated bienzyme（A-G@BS） exhibits enhanced stabilities of thermal, pH, recycling and tolerance of xylene. The A-G@BScontaining coating releases H2O2 at rates exceeding a target of 36 nmol·cm-2·d-1for 90 days in a laboratory assay. The results demonstrate that the method is a promising coating technology for entrapping active enzymes,presenting an interesting avenue for enzyme-based antifouling solutions.

关 键 词：Antifouling Bienzyme H2O2 Coating 

分 类 号：TQ637[化学工程—精细化工] TQ929[轻工技术与工程—发酵工程]