冰亲和吸附装置对胶原抗冻肽的分离纯化  

Purification of antifreeze collagen peptides by ice affinity adsorption protocol

作　　者：阮功成[1] 曹慧[1] 徐斐[1] 于劲松[1] 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《食品与发酵工业》2015年第9期19-23,共5页Food and Fermentation Industries

基　　金：国家自然科学基金青年基金(31201421);国家高技术研究发展计划(863计划)项目(2013AA102207)

摘　　要：抗冻肽是一类可以非依数性降低体系冰点的多肽,在生命体内具有非常重要的生理作用。研究搭建了冰亲和吸附装置,并以微生物保护活性为指标,优化了其对猪皮胶原酶解复合物中胶原抗冻肽的吸附条件,同时对分离产物的性质进行了研究。结果表明:在胶原酶解复合物浓度为1 mg/mL,吸附时间为10 h,吸附温度为-5℃,吸附次数为2次的条件下,所获得的胶原抗冻肽对微生物的低温保护活性最强。经过冰亲和吸附后,胶原抗冻肽的主要洗脱峰之间的分离效果明显增加,其胶原抗冻肽的分子质量主要分布小于1 000 Da、且富含甘氨酸、脯氨酸和羟脯氨酸。Antifreeze collagen peptides is a family of peptides which can lowering the freezing point of the system non-colligatively. This plays an important role in protecting organisms from freezing injury and damage. In this study, we studied the ice affinity adsorption equipment, used micro-organisms protection as the index to optimize the condition of purifying pig skin collagen peptides by enzymatic hydrolysis. The optimum conditions were： crude mixture concentration of 1 mg/mL, at -5 ℃ for 10 hour, adsorption twice. After ice affinity adsorption, collagen peptides peaks had better separation effect, the molecular weight mainly distributed within the range of 1000 Da, and major collagen amino acids were Proline and Hydroxyproline.

关 键 词：胶原酶解复合物 胶原抗冻肽 吸附 冰亲和吸附装置 分子质量分布 

分 类 号：TS201.2[轻工技术与工程—食品科学]