The integration characteristics of the exogenous growth hormone gene in a transgenic common carp(Cyprinus carpio L.) with fast-growth performance  

作　　者：Ji Chen Qing Luo Hairong Bao Lanjie Liao Yongming Li Zuoyan Zhu Yaping Wang Wei Hu 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences [2]University of Chinese Academy of Sciences

出　　处：《Science Bulletin》2015年第19期1654-1660,共7页科学通报（英文版）

基　　金：supported by the National High Technology Research and Development Program of China(‘‘863’’Program)(2011AA100404,2011AA100403);the National Natural Science Foundation of China(31325026,31272649)

摘　　要：The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an ‘‘all-fish'' growth hormone(GH)transgenic common carp family that exhibits fast growth.In this present study, genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an ATrich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site.The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an ＂all-fish＂ growth hormone （GH） transgenic common carp family that exhibits fast growth. In this present study, genome walking, real-time quantita- tive polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an AT- rich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site.

关 键 词：Common carp Growth hormone TRANSGENE Integration site Copy number 

分 类 号：S917.4[农业科学—水产科学]