Human induced pluripotent stem cells labeled with fluorescent magnetic nanoparticles for targeted imaging and hyperthermia therapy for gastric cancer  

作　　者：Chao Li Jing Ruan Meng Yang Fei Pan Guo Gao Su Qu You-Lan Shen Yong-Jun Dang Kan Wang Wei-Lin Jin Da-Xiang Cui 

机构地区：[1]Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education,Department of Instrument Science and Engineering, National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University [2]Basic Medical Sciences Department of Biochemistry &Molecular Biology Key Laboratory of Molecular Medicine, Fudan University [3]Department of Imaging and Nuclear Medicine, Shanghai Sixth People's Hospital

出　　处：《Cancer Biology & Medicine》2015年第3期163-174,共12页癌症生物学与医学（英文版）

基　　金：supported by National Natural Science Foundation of China (Grant No. 81225010, 20803040, 81028009, and 31170961);National Key Basic Research Program of China (973 Program) (Grant No. 2010CB933902 and 2015CB931802);National Key Technology Research and Development Program (863 Program) (Grant No. 2012AA022703 and 2014AA020700);Shanghai Science and Technology Fund (Grant No.13NM1401500)

摘　　要：Objective: Human induced pluripotent stem(i PS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human i PS cells labeled with fluorescent magnetic nanoparticles(FMNPs) for targeted imaging and synergistic therapy of gastric cancer cells in vivo. Methods: Human i PS cells were prepared and cultured for 72 h. The culture medium was collected, and then was coincubated with MGC803 cells. Cell viability was analyzed by the MTT method. FMNP-labeled human i PS cells were prepared and injected into gastric cancer-bearing nude mice. The mouse model was observed using a small-animal imaging system. The nude mice were irradiated under an external alternating magnetic field and evaluated using an infrared thermal mapping instrument. Tumor sizes were measured weekly. Results: iP S cells and the collected culture medium inhibited the growth of MGC803 cells. FMNP-labeled human iP S cells targeted and imaged gastric cancer cells in vivo, as well as inhibited cancer growth in vivo through the external magnetic field. Conclusion: FMNP-labeled human i PS cells exhibit considerable potential in applications such as targeted dual-mode imaging and synergistic therapy for early gastric cancer.Objective： Human induced pluripotent stem （iPS） cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human iPS cells labeled with fluorescent magnetic nanoparticles （FMNPs） for targeted imaging and synergistic therapy of gastric cancer cells in vivo. Methods： Human iPS cells were prepared and cultured for 72 h. The culture medium was collected, and then was co- incubated with MGC803 cells. Cell viability was analyzed by the MTT method. FMNP-labeled human iPS cells were prepared and injected into gastric cancer-bearing nude mice. The mouse model was observed using a small-animal imaging system. The nude mice were irradiated under an external alternating magnetic field and evaluated using an infrared thermal mapping instrument. Tumor sizes were measured weekly. Results： iPS cells and the collected culture medium inhibited the growth of MGC803 cells. FMNP-labeled human iPS cells targeted and imaged gastric cancer cells in vivo, as well as inhibited cancer growth in vivo through the external magnetic field. Conclusion： FMNP-labeled human iPS cells exhibit considerable potential in applications such as targeted dual-mode imaging and synergistic therapy for early gastric cancer.

关 键 词：Human induced pluripotent stem cell （human iPS cells） targeted imaging hyperthermia therapy fluorescent magneticnanoparticles gastric cancer nude mice 

分 类 号：R735.2[医药卫生—肿瘤]