利用噬菌体随机肽库对神经胶质瘤母系SWO-38进行全细胞筛选  

Whole cell screening of phage-display peptide library for mimicry peptides of glioma SWO-38

作　　者：高双荣[1] 钟雪云[1] 颜艳灵[1] 钟振宇[1] 秦艳芳[1] 孙艳花[1] 王冰[1] 于莉娜[1] 钟瑛[1] 

机构地区：[1]暨南大学医学院病理学教研室,广东广州510632

出　　处：《中国病理生理杂志》2002年第8期946-948,共3页Chinese Journal of Pathophysiology

基　　金：广州市科技局科研重点立项课题 (No1 .2 0 0 1 -Z - 0 1 - 2 );广东省科技厅研究团队项目课题 (No2 .0 1 50 1 2 )

摘　　要：目的和方法 :用噬菌体随机 7肽库对神经胶质瘤母细胞 (SWO - 38)进行全细胞筛选 ,并通过竞争结合实验检验所筛选出的克隆的结合特异性。结果 :经过 3轮“吸附 -洗脱 -扩增” ,噬菌体高度富集 ,并得到两个特异性较强的克隆。结论 :利用噬菌体肽库可以简便。AIM and METHODS:The Ph.D.-7 phage display library was used to isolate peptides specific for glioma SWO-38 cell by whole cell screening.Moreover,binding efficiency analysis was carried out to test the binding specificity of the clones obtained. RESULTS: After three rounds of biopanning,a high concentration of phage clones was obtained and two of them were found to be highly specific to glioma SWO-38. CONCLUSION: Highly specific clones against neurtral glioma cells can be obtained from a phage display library by simple procedures.

关 键 词：噬菌体随机肽库 全细胞筛选 噬菌体 神经胶质瘤 肿瘤导向治疗 

分 类 号：R730.264[医药卫生—肿瘤]