RNA干扰沉默USP22基因对人鼻咽癌裸鼠移植瘤生长的影响  

Effect of RNAi-mediated USP22 gene silencing on the growth of human nasopharyngeal carcinoma xenograph in nude mice

作　　者：廖志伟[1] 庄雅靖 余宏伟[1] 喻芳[1] 刘孟忠[2] 周同冲[1] 

机构地区：[1]广州医科大学附属肿瘤医院放疗科,广东广州510095 [2]中山大学肿瘤防治中心放疗科,广东广州510060

出　　处：《中国医药导报》2015年第30期25-28,45,F0003,共6页China Medical Herald

基　　金：广东省广州市医药卫生科技项目(20141A011092);广州医学院青年科研项目(2012A15)

摘　　要：目的探讨USP22基因表达沉默对人鼻咽癌裸鼠移植瘤生长的影响。方法将9只BALB/C祼鼠随机分为阴性对照组、CNE-2空载体细胞株组(Scrambled组)、USP22表达下调稳定细胞株组(sh-USP22组),每组3只裸鼠。绘制各组肿瘤生长曲线;移植瘤中USP22 m RNA及蛋白的表达情况通过RT-PCR、免疫组织化学法检测。结果 sh-USP22组肿瘤重量[(0.54±0.07)g]明显小于阴性对照组[(1.58±0.24)g]和Scrambled组[(1.46±0.23)g],差异均有统计学意义(均P<0.05);RT-PCR结果表明,以阴性对照组表达量为参照(1),sh-USP22组肿瘤组织中USP22m RNA相对表达量(0.14±0.09)低于阴性对照组和Scrambled组(0.92±0.11),差异均有统计学意义(均P<0.05);而阴性对照组和Scrambled组(0.92±0.11)比较,差异无统计学意义(P>0.05);免疫组化结果表明,sh-USP22组肿瘤组织中USP22阳性细胞所占比率[(12.45±1.16)%]明显低于阴性对照组[(97.25±1.94)%]和Scrambled组[(91.28±2.75)%],差异均有统计学意义(均P<0.05);阴性对照组[(97.25±1.94)%]和Scrambled组[(91.28±2.75)%]比较,差异无统计学意义(P>0.05)。结论慢病毒载体sh-USP22能有效抑制USP22基因表达,并有效抑制CNE-2细胞的裸鼠成瘤能力,USP22有望成为鼻咽癌基因治疗新靶点。Objective To study the effect of USP22 gene silencing by RNA interference on the growth of human na- sopharyngeal carcinoma growth in nude mouse. Methods 9 cases of BALB/c nude mice were randomly divided into negative control group （n=3）,the Scrambled group （n=3） and the sh-USP22 group （n=3）. The curve of tumor growth was then described; USP22 mRNA and protein expressions were detected by RT-PCR and inmunohistochemistry. Results The weight of tumor in sh-USP22 group [（0.54±0.07）g] was lighter than negative control group [（1.58±0.24）g] and scrambled group [（1.46±0.23）g], and the differences were statistically significant （all P 〈 0.05）. According to the results of RT-PCR, the USP22 mRNA expression in negative control group as a reference （1）, the sh-USP22 group （0.14±0.09） was significantly lower than that in the negative control group and the scrambled group （0.92±0.11）, and the differences were statistically significant （all P 〈 0.05）. Furthermore, the expression of USP22 positive cells of sh-USP22 group [（12.45±1.16）%] was also significantly lower than the negative control group [（97.25±1.94）%] and the scrambled group [（91.28±2.75）%], and the differences were Statistically significant（all P 〈 0.05）. Conclusion RNA interference targeting USP22 inhibits the expression of USP22 and the growth of nasopharyngeal carcinoma in nude mice. USP22 may be a target for gene therapy in nasopharyngeal carcinoma.

关 键 词：鼻咽癌 CNE-2 细胞 USP22 RNA干扰 裸鼠移植瘤 

分 类 号：R739.63[医药卫生—肿瘤]