ITGA2 C807T多态性与大肠腺瘤及大肠癌发病风险的相关性  

Association of ITGA2 C807T polymorphism with risk of colorectal adenoma and colorectal cancer

作　　者：李佳玲[1] 陈杰[2] 韩明[1] 刘立玺[1] 龚敏[1] 李兴[1] 文萍[1] 刘爱莲[1] 秦章禄[3] 韩容芬 文剑波[1] 

机构地区：[1]南方医科大学附属萍乡医院消化内科,江西省萍乡市337000 [2]南方医科大学附属萍乡医院药学部,江西省萍乡市337000 [3]南方医科大学附属萍乡医院胃肠外科,江西省萍乡市337000

出　　处：《世界华人消化杂志》2015年第27期4358-4367,共10页World Chinese Journal of Digestology

基　　金：江西省科技支撑计划项目;No.20141BBG70071~~

摘　　要：目的:探讨中国江西汉族人群ITGA2 C807T基因多态性与大肠腺瘤及大肠癌患病风险的关系.方法:本研究为基于医院人群的病例-对照研究,包括95例正常对照、48例大肠腺瘤和89例大肠癌个体.采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction restriction fragment length polymorphism assay,PCR-RFLP)方法检测研究人群ITGA2C807T基因型分布,并对该基因多态性与大肠癌临床病理特征的关系进行分析.结果:ITGA2 C807T基因型频率和等位基因频率在大肠腺瘤组和对照组的总体分布无显著差异,而其在大肠癌组和对照组的分布具有显著差异.相比于野生基因型CC,携带变异基因型(CT+TT)的个体患大肠腺瘤和大肠癌的风险均增高.在分层分析中,变异基因型并未增加患大肠腺瘤的风险,变异基因型增加患大肠癌的风险在女性、吸烟、饮酒、高学历、脑力劳动、城市受试者中具有统计学意义.大肠癌病例分别按病变部位、病理类型、肿瘤大小、分化程度、浸润深度、淋巴结转移和Dukes分期临床病理特征分层后,均未见ITGA2 C807T基因型分布存在显著性差异.结论:ITGA2 C807T基因多态性可能增加大肠腺瘤和大肠癌患病风险.AIM：To evaluate the impact of the ITGA2 C807 T gene polymorphism on the risk of colorectal adenoma（CRA） and colorectal cancer（CRC） in a Chinese Han population.METHODS：A hospital-based case-control study was conducted,including 95 healthy controls,48 patients with CRA and 89 patients with CRC.Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）assay.Association of the genotypes with the clinical and pathological features of CRC was evaluated.RESULTS：The frequencies of the genotypes and alleles in CRC were significantly different from those of controls,but there were no significant differences between CRA and controls.Compared with individuals with the wild type genotype CC,subjects with the variant genotypes（CT＋TT） had a significantly higher risk of CRA and CRC.In stratified analyses,the elevated CRC risk was especially evident in older individuals,females,smokers,drinkers,well-educated subjects,mental workers and urban subjects.However,no correlation was observed between CRA patients and controls in stratified analyses.When stratified by clinicopathological features such as lesion distribution,pathology subtype,tumor size,differentiation degree,depth of invasion,lymph node metastasis and Duke＇s stage in patients with CRC,no associations were observed in the polymorphism distributions.CONCLUSION：The ITGA2 C807T polymorphism may be associated with an increased risk of CRA and CRC.

关 键 词：ITGA2 基因多态性 大肠腺瘤 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]