siRNA下调RIPK4基因表达对人成骨肉瘤MG-63细胞增殖的抑制作用  

Inhibition of proliferation of human osteosarcoma MG-63 cells by siRNA-induced down-regulation of expression of RIPK4 gene

作　　者：田夏威 蒋伟龙[1] 姜金[1] 胡旭昌[1] 杨明轩[1] 汪静[1] 安丽萍[1] 马静琳 王栓科[1] 

机构地区：[1]兰州大学第二医院骨科,甘肃省骨关节疾病研究重点实验室,甘肃兰州730030

出　　处：《肿瘤》2015年第10期1070-1075,共6页Tumor

基　　金：甘肃省基础研究创新群体项目(编号:1308RJIA004)~~

摘　　要：目的:探讨si RNA抑制受体相互作用蛋白激酶4(receptor-interacting protein kinase 4,RIPK 4)基因表达对人成骨肉瘤MG-63细胞增殖及细胞内Wnt/β-catenin信号通路相关基因表达的影响。方法 :将靶向RIPK4基因的si RNA(RIPK4 si RNA)转染入人成骨肉瘤MG-63细胞后,应用CCK-8法检测MG-63细胞的增殖能力,实时荧光定量PCR法和蛋白质印迹法检测细胞内RIPK4、β-catenin和cyclin D1m RNA及蛋白的表达水平。结果 :RIPK4 si RNA转染后,MG-63细胞的增殖能力显著低于阴性对照组(MG-63细胞转染control si RNA)和空白对照组(未转染任何si RNA的MG-63细胞)(P值均<0.05);RIPK4 si RNA转染组细胞中RIPK4、β-catenin和cyclin D1 m RNA及蛋白的表达水平显著低于阴性对照组和空白对照组(P值均<0.05)。结论 :沉默RIPK4基因的表达可以抑制人成骨肉瘤MG-63细胞的增殖,这一作用可能与抑制Wnt/β-catenin信号通路有关。Objective： To investigate the effect of siRNA-induced down- regulation of receptor-interacting protein kinase 4 （RIPK4） gene on proliferation of human osteosarcoma MG-63 cells and expressions of Wnt/β-catenin signaling pathway-associated genes. Methods： The ostersarcoma MG-63 cells were transfected with siRNA targeting RIPK4 gene （RIPK4 siRNA）, then the proliferation of MG-63 cells was examined by CCK-8 method, and the mRNA and protein expression levels of RIPK4, β-catenin and cyclin D1 were detected byreal-time fluorescent quantitative-PCR and Western blotting, respectively Results： The proliferative ability of MG-63 cells after transfection with RIPK4 siRNA was lower than that of MG-63 cells transfected with the control siRNA （as a negative control） or without any transfection （as a blank control） （both P 〈 0.05）. The mRNA and protein expression levels of RIPK4, β-catenin and cyclin D1 in RIPK4 siRNA transfection group were lower than those of the negative control and the blank control groups （all P 〈 0.05）. Conclusion： Silencing the expression of RIPK4 gene can inhibit the proliferation of MG-63 cells. This effect may be related to the inhibition of Wnt/β-catenin signaling pathway.

关 键 词：骨肉瘤 RNA 小分子干扰 细胞增殖 MG-63细胞 

分 类 号：R738.1[医药卫生—肿瘤]