基于二氧化硅包裹三联吡啶钌的新型咖啡因电致化学发光传感器  

A novel electrochemiluminescence caffeine sensor based on a Tris(2,2'-bipyridyl) dichlororuthenium(Ⅱ)doped Silica/Nafion film modified electrode

作　　者：李晓宇[1] 谭学才[1] 严军[1] 胡琪[1] 吴佳雯[1] 张慧[1] 陈晓[1] 

机构地区：[1]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西高校食品安全与药物分析化学重点实验室,南宁530008

出　　处：《分析试验室》2015年第10期1127-1131,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21365004);广西自然科学基金重点项目(2013GXNSFDA019006);广西高等学校高水平创新团队及卓越学者资助计划(桂教人[2014]7号);广西民族大学研究生创新基金(GXUN-chx2014096)项目资助

摘　　要：将包裹了三（2,2＇-联二吡啶）二氯化钌（Ⅱ）配合物（Ru（bpy）32＋）的二氧化硅纳米粒子（Ru（bpy）32＋@Si O2）通过Nafion膜修饰在玻碳电极上,并研究了咖啡因在此电极上的电化学发光行为。在单因素实验的基础上,结合响应面分析法对实验条件进行了优化,建立了一种检测咖啡因的新方法。在最佳的实验条件下,咖啡因浓度在5.0×10-3~20μmol/L范围内与其相对发光强度呈良好的线性关系（r2=0.9996）,检出限（S/N=3）为1.3×10-3μmol/L。连续平行测定1μmol/L的咖啡因溶液5次,相对发光强度的RSD为3.8%。方法用于可乐样品的实际测定,回收率为93.7%~103.8%。A novel method for determination of caffeine using electrochemiluminescence（ ECL） based on Tris（ 2,2＇-bipyridyl） dichlororuthenium（ Ⅱ）-doped silica（ Ru（ bpy）32 ＋@ Si O） / Nafion film modified electrode is presented in this paper. These Ru（ bpy）32 ＋@ Si Onanoparticles were prepared by water-in-oil microemulsion method,then characterized by scanning electron microscopy. The electrochemical test showed that Ru（ bpy）32 ＋@ Si O2 nanoparticles maintained its high ECL efficiency. The single factor experiment was combined with response surface methodology to optimize the experimental condition. Under the optimal experimental condition,it has good linearity in the concentration range from 5. 0 × 10- 3~ 20 μmol / L（ r2= 0. 9996） with the detection limit of 1. 3 × 10- 3μmol /L. The RSD of ECL intensity for five-time determination of 1 μmol /L caffeine was 3. 8%. This method has been successfully applied to determine caffeine in cola with the recovery from 93. 7% ~ 103. 8 %.

关 键 词：电化学发光 联吡啶钌 硅溶胶 咖啡因 响应面 

分 类 号：O657.3[理学—分析化学]