尿常规联合显微镜尿红细胞形态检查在Alport综合征早期诊断中的应用  

Evaluation of routine urine check with optical microscopy measuring urinary erythrocyte morphology in early diagnosis of Alport syndrome in Seven families

作　　者：付荣[1] 吴清岩[1] 刘娟[1] 缑梦帆 同少峰[1] 和俊杰[1] 

机构地区：[1]濮阳市油田总医院儿科,河南濮阳457001

出　　处：《新乡医学院学报》2015年第9期864-867,共4页Journal of Xinxiang Medical University

摘　　要：目的探讨尿常规联合普通光学显微镜尿红细胞形态检查在早期发现和诊断Alport综合征（AS）家系中的应用。方法对门诊和住院患儿尿常规检查发现红细胞尿者,在1周内进行1~2次复查,若复查仍为红细胞尿者,取其晨起新鲜尿液10 m L,1 500 r·min-1离心10 min,弃上清液,光学显微镜下观察尿沉渣红细胞形态。如红细胞为严重变形红细胞,诊断为肾性血尿,对其父母行尿自动分析检查及尿红细胞形态检查,有异常者进行家系调查。结果通过尿常规检查联合普通光学显微镜尿红细胞形态检查,发现7个肾性血尿患儿,经过家系调查初步诊断为X连锁显性遗传AS家系。结论尿常规联合普通光学显微镜尿红细胞形态检查能够早期发现和诊断AS家系,值得在基层医院应用。Objective To discuss the application of routine urine check with optical microscopy measuring urinary erythrocyte morphology to the early discovery and diagnosis of Alport syndrome（ AS） family. Methods One to two rechecks within one week were conducted for the children in the outpatient and the hospitalization patients who were found to have urine erythrocyte. If the recheck result still proved that the children had urine erythrocyte,10 m L fresh urine in the early morning of the children were collected. The supernatant was removed through centrifugal rotation at a speed of 1 500 rotations per min for ten minutes,and the supernatant was outwell after that. The erythrocyte morphology of the urine sediment was observed under the optical microscope. If the erythrocyte proved to be seriously deformed,the child was diagnosed as renal hematuresis and the child＇s parents had urine autoanalysis check and erythrocyte morphological check. If the check proved to be abnormal,a family survey was needed. Results Seven children were discovered to have urine hematuresis through the routine urine check and urine erythrocyte morphological check. The seven family were diagnosed primarily as the X-chain dominant inheritance AS family though family survey. Conclusion Routine urine test and erythrocyte morphological check based on the ordinary optical microscope can discover and diagnose the AS family at an earlier date. It is especially worthy to be application in the grassroots hospitals.

关 键 词：尿红细胞形态 ALPORT综合征 家系 诊断 

分 类 号：R726.9[医药卫生—儿科]