Assessment of Bisphenol A(BPA) neurotoxicity in vitro with mouse embryonic stem cells  

作　　者：Nuoya Yin Xinglei Yao Zhanfen Qin Yuan-Liang Wang Francesco Faiola 

机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences [2]Section of Molecular Biology,University of California at San Diego

出　　处：《Journal of Environmental Sciences》2015年第10期181-187,共7页环境科学学报（英文版）

基　　金：supported by a Chinese Academy of Sciences(CAS)Strategic Leading Science&Technology Program grant(XDB14040301);by the Hundred Talent Program of CAS(121311ZXPP2014004)at the Research Center for Eco-Environmental Sciences(RCEES),CAS

摘　　要：The adverse effects of environmental pollution on our well-being have been intensively studied with many in vitro and in vivo systems. In our group, we focus on stem cell toxicology due to the multitude of embryonic stem cell（ESC） properties which can be exerted in toxicity assays. In fact, ESCs can differentiate in culture to mimic embryonic development in vivo, or specifically to virtually any kind of somatic cells. Here, we used the toxicant Bisphenol A（BPA）, a chemical known as a hazard to infants and children, and showed that our stem cell toxicology system was able to efficiently recapitulate most of the toxic effects of BPA previously detected by in vitro system or animal tests. More precisely, we demonstrated that BPA affected the proper specification of germ layers during our in vitro mimicking of the embryonic development, as well as the establishment of neural ectoderm and neural progenitor cells.The adverse effects of environmental pollution on our well-being have been intensively studied with many in vitro and in vivo systems. In our group, we focus on stem cell toxicology due to the multitude of embryonic stem cell（ESC） properties which can be exerted in toxicity assays. In fact, ESCs can differentiate in culture to mimic embryonic development in vivo, or specifically to virtually any kind of somatic cells. Here, we used the toxicant Bisphenol A（BPA）, a chemical known as a hazard to infants and children, and showed that our stem cell toxicology system was able to efficiently recapitulate most of the toxic effects of BPA previously detected by in vitro system or animal tests. More precisely, we demonstrated that BPA affected the proper specification of germ layers during our in vitro mimicking of the embryonic development, as well as the establishment of neural ectoderm and neural progenitor cells.

关 键 词：Bisphenol A（BPA） Stem cell toxicology Neurotoxicity Embryonic stem cells（ESCs） Developmental toxicity Neural ectoderm 

分 类 号：X171.5[环境科学与工程—环境科学]