柴胡皂苷d在肝癌模型大鼠体内的药效学评价  

Efficacy evaluation of saikosaponin d in treatment of hepatoma model rat in vivo

作　　者：王维[1] 李海林[1] 韩光宁 宋国权[1] 代海平[2] 彭永海[3] 

机构地区：[1]牡丹江医学院红旗医院普外二科,黑龙江牡丹江157011 [2]牡丹江医学院红旗医院病理科,黑龙江牡丹江157011 [3]南京军区福州总医院肿瘤科,南京210000

出　　处：《中国临床药理学杂志》2015年第20期2027-2030,共4页The Chinese Journal of Clinical Pharmacology

基　　金：福建省自然科学基金资助项目(2009J01184)

摘　　要：目的评价柴胡皂苷d在二乙基亚硝胺诱发的肝癌模型大鼠体内的药效学。方法将60只建模成功的SD大鼠随机分为模型组、实验组及对照组,每组20只;20只未处理的SD大鼠为安慰剂组。实验组予以腹腔注射10mg·kg-1柴胡皂苷d注射液,每日1次;对照组予以腹腔注射200 mg·kg-1沙利度胺注射液,每日1次;模型组和安慰剂组均腹腔注射0.9%氯化钠注射液2m L,每日1次。所有干预均连续6周。干预结束后,用生化分析法检测大鼠肝功能指标,用免疫组化法检测增殖细胞核抗原(PCNA)和MKI-67基因编码核蛋白(Ki-67)表达,用HE染色法观察肝组织病理学形态。结果与模型组相比,实验组与对照组的肝功能指标较低(P<0.05),PCNA和Ki-67表达较低(P<0.05)。与对照组相比,实验组中各项肝功指标较低(P<0.05),PCNA和Ki-67表达较低(P<0.05)。安慰剂组的肝病理切片显示正常的肝小叶结构完整,肝索周围规整,肝细胞索排列整齐,细胞核清晰。模型组显示癌灶呈现片状坏死,正常肝小叶结构被破坏,白色结节处为肝癌细胞。对照组仍能观察到少量恶性度较低以及局灶性坏死的癌细胞,并且有较少的核分裂象,实验组的肝组织肝血窦中有少量充血,极少的核分裂象。结论柴胡皂苷d能显著改善肝癌模型大鼠的肝功能,修复损伤的肝细胞,降低肝癌模型大鼠PCNA和Ki-67蛋白的表达量。Objective To evaluate the pharmacodynamics of saikosapo- nin d （SSd）in the treatment of hepatoma model rat. Methods Sixty SD rats with succeed modeling were randomly divided into model group （ n = 20） , experimental group （ n = 20） and control group （ n = 20） , 20 SD rats without modeling were conducted to placebo group. The experi- mental group was treated with SSd 10 mg~ kg-1 once a day, the control group was treated with thalidomide 200 mg~ kg-~ once a day, the place- bo group and model group were treated with 0. 9% NaCI 2 mL once a day. Liver function index were compared by Biochemical analysis me- thod, proliferating cell nuclear antigen （PCNA） and MKI - 67 gene en- codes nuclear protein（Ki-67） were compared by immunohistochemistry after 6 weeks. Results Compared with the model group, liver function index as well as the expression of PCNA and Ki -67 of the control group and the experiment group were lower （P 〈0.05）. Compared with the control group, liver function index with expression of PCNA and Ki -67 of the experiment group were lower （P 〈0. 05）. Pathological display： the pathological sections of the liver in the placebo group showed that the structure of the normal liver was complete, the liver cells were neat, the liver cells were arranged regularly, and the nu- cleus was clear. The model group showed that the tumor foci were in the shape of the necrosis, and the structure of the normal liver was destroyed, and the white nodules were the liver cancer cells. A small amount of low malignant degree, focal necrosis of cancer ceils and fewer mitoses could be observed in control group, a small amount of blood and rare mitotic figures could be seen in the experiment group. Conclusion SSd can significantly improve liver function, repair the damage of liver cells, and reduce protein expression of PCNA and Ki - 67 in model rats.

关 键 词：柴胡皂苷D 肝癌 增殖细胞核抗原 MKI-67基因编码核蛋白 

分 类 号：R735.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]