恒强旋转磁场对骨折愈合过程中骨形态蛋白-2和血小板源性生长因子表达的影响  

The effect of rotation constancy static magnetic field on the expression of BMP-2 and PDGFs during fracture healing

作　　者：李海伟[1,2] 李玉虎[3] 刘海涛[2] 高维纬[4] 

机构地区：[1]临汾山西师范大学体育学院,041000 [2]北京体育大学研究生院 [3]湛江国家体育总局湛江潜水运动学校 [4]北京体育大学运动医学教研室

出　　处：《中华物理医学与康复杂志》2015年第10期721-726,共6页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：中央高校基本科研业务费专项资金资助（2012BS009）

摘　　要：目的观察恒强旋转磁场对骨折愈合过程中骨形态蛋白-2（BMP-2）和血小板源性生长因子（PDGFs）在骨折部位不同时间表达的变化，探讨恒强旋转磁场促进骨折愈合的机制。方法将80只建立股骨闭合性骨折模型的Sprague Dawley（SD）大鼠按随机数字表法分为磁场干预组和对照组，每组40只。磁场干预组大鼠置于0．4T恒强旋转磁场（浙江康尔医疗器械公司）中，磁体旋转频率6Hz，每日磁场干预1次，每次连续30min，每周6次；对照组未做任何干预处理。分别于骨折后2、3、4和8周四个观察时间点（每个时间点10只大鼠），观察各组大鼠骨折部位BMP-2、PDGF—A和PDGF—B免疫组化染色阳性表达，并采用Nikon显微成像分析仪（Nikon 50i，日本）拍照（图像放大400倍），用生物图像分析处理软件测量阳性染色区域的积分光密度值（IOD）。采用双因素方差分析比较时间、磁场的主效应及二者的交互效应。结果①不同时间点各组大鼠骨折部位BMP-2阳性表达的IOD值比较，差异有统计学意义（F=21．58，P〈0．01），多重比较显示，BMP-2阳性染色区域在骨折后2、3和4周时的IOD值均明显高于骨折后8周（P〈0．01），骨折后4周的IOD值亦明显高于骨折后2周（P〈0．01）；磁场干预与时间存在有交互作用（F=3．17；P〈0．05）。②磁场干预组与对照组大鼠不同时间点间骨折部位的PDGF-A阳性表达IOD比较，差异有统计学意义（F=28．16，P〈0．01），多重比较显示，骨折后2周骨折部位PDGF—A的阳性表达的IOD值显著高于骨折后3周（P〈0．01）、4周（P〈0．01）和8周（P〈0．01），骨折后3周和4周PDGF-A的阳性表达IOD也显著高于骨折后8周（P〈0．01）。③磁场干预组骨折部位的PDGF—B阳性表达IOD值明显高于对照组[磁场干预组（57．6±2．1）vs对照组（50．11±2．22）]，差异有统计学意义（F=5．98，P〈0．0Objective To evaluate the effect rotation constancy static magnetic field（RCSMF） on the expression of bone morphogenetic protein-2 （BMP-2） and platelet-derived growth factors （PDGFs） in fracture healing, and elucidate the possible mechanism of RCSMF promoting fracture healing. Methods A total of 80 rats with femur closed fracture were randomly divided into a magnetic treatment group （M） and a control group （C）. The M group was given RCSMF treatment 30 minutes per day, 6 times per week, using 0.4 T 6 Hz magnetic field, while the C group was not given any intervention. Femurs and calluses were subjected to BMP-2, PDGF-A and PDGF-B immunohistochemistry assay at 2, 3, 4 and 8 weeks after the treatment. The integrated optical density （IOD） of positive staining was calculated. Two-factor variance analysis was used to compare the main effect of time, treatment and that of their interaction. Results For both groups, the IOD of immunoreactivity of BMP-2 at 2, 3 and 4 weeks after the treatment was significantly higher than that 8 weeks after the treatment, and the value at four weeks after the intervention was also significantly higher than that at 2 weeks after it. There was a significant interaction effect of time × treatment of BMP-2 （ F = 3.17, P 〈 0.05 ）. Significant differences were observed in the IOD of positive staining PDGF-A at different time points for both groups ： the value at 2 weeks after treatment was significantly higher than that at 3, 4 and 8 weeks after the treatment, and that at 3 and 4 weeks after treatment was also significantly higher than that at 8 weeks after the treatment. The IOD of positive staining PDGF-B of the M group （57.6 ±2.1 ） was significantly higher than that of the C group （50.11 ±2.22, P 〈 0.05）. There was significant difference in the IOD of positive staining PDGF-B （F =50.06,P 〈0.01） at different time points： the IOD of positive staining PDGF-B at 3 weeks after treatment was significantly higher than that at 2 and 8 weeks group

关 键 词：磁场 骨折 骨形态蛋白 血小板源性生长因子 

分 类 号：R[医药卫生]