感染后肠易激综合征小鼠肠道热休克蛋白70的表达及意义  

Expression of heat shock protein 70 in intestinal tissue of mice with post-infectious irritable bowel syndrome

作　　者：周旭春[1] 孙晓宁[2] 杨波[1] 黄白丽[2] 邓桃枝[2] 何周桃[2] 韩向阳[2] 蓝程[2] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆市400046 [2]海南省人民医院消化内科,海南省海口市570311

出　　处：《世界华人消化杂志》2015年第26期4177-4183,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81160057;海南省国际科技合作专项基金资助项目;No.KJHZ2013-14~~

摘　　要：目的:探讨感染后肠易激综合征(postinfectious irritable bowel syndrome,PI-IBS)小鼠肠道热休克蛋白70(heat shock protein 70,HSP70)表达及其与肠道低度炎症的相关性.方法:旋毛虫感染制作PI-IBS小鼠模型.免疫组织化学检测PI-IBS小鼠肠道HSP70蛋白质表达.肠道组织HE染色,Dieleman LA肠道炎症评分系统评分,腹壁撤退反射(abdominal withdrawal reflex,AWR)检测内脏高敏感性,结肠传输试验(colon transportation test,CTT)观察其肠道动力改变.Spearman分析肠道HSP70表达与炎症评分、AWR及CTT的相关性.结果:感染旋毛虫第8周,PI-IBS小鼠肠道炎症评分高于对照组但无显著性差异(P>0.05).PI-IBS小鼠回肠末段HSP70蛋白质表达较对照组明显升高(P<0.01),但结肠未见明显变化(P>0.05).PI-IBS小鼠回肠HSP70蛋白质表达与肠道炎症评分呈弱负相关(P>0.05),与AWR和Bristol评分呈显著负相关(P<0.01),与.结肠HSP70表达与AWR及Bristol评分均无明显相关性(P>0.05).结论:PI-IBS时肠道存在低度炎症,回肠末段HSP70的表达异常可能与肠道低度炎症、内脏高敏感性和肠道动力改变有关.AIM：To detect the expression of heat shock protein 70（HSP70） in intestinal tissue of mice with post-infectious irritable bowel syndrome（PI-IBS）.METHODS：A mouse model for PI-IBS was established by infection of mice with trichinella spiralis in vivo.The inflammatory score of the intestine of PI-IBS mice was calculated,and abdominal withdrawal reflex（AWR） and colon transportation test（CTT） were used to evaluate the clinical features of the PI-IBS mice.Intestinal expression of HSP70 was measured by immunohistochemistry.Spearman correlation analysis was carried out to assess the relationship between the expression of HSP70 and the clinical features of PI-IBS.RESULTS：The PI-IBS mouse model was established successfully.On the 8th week,there was no visible inflammation in the intestine（ileal inflammation score：P〉0.05）,whereas the clinical features significantly changed,including increased AWR score（P〈0.01） and decreased CTT score（P〈0.01）.The expression of HSP70 significantly increased in the ileum but not in the colon of PI-IBS mice at the 8th week（ileum：P〈0.01;colon：P〉0.05）.The expression of HSP70 in the ileum,but not in the colon,was remarkably related with the clinical features of PI-IBS mice,including the negative relationship with AWR（P〈0.01）,and the positive relationship with CTT（P〈0.01）.CONCLUSION：HSP70 might be associated with low-grade intestinal inflammation induced visceral hypersensitivity and abnormal intestinal motility in mice with PI-IBS.

关 键 词：热休克蛋白70 感染后肠易激综合征 炎症 内脏高敏感性 肠道动力 

分 类 号：R574.4[医药卫生—消化系统]