马铃薯内生拮抗真菌E-19的分离、鉴定及其次生代谢产物研究  

Isolation and Identification of Secondary Metabolites of Endophytic Fungi from Solanum tuberosum

作　　者：杨曼思 王文祥[1] 李龙根[1] 艾洪莲[1] 郭华春[1] 

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201

出　　处：《天然产物研究与开发》2015年第10期1728-1731,1736,共5页Natural Product Research and Development

基　　金：国家马铃薯产业技术体系(CARS-10);云南省自然科学基金(2013ZA007)

摘　　要：采用组织分离法,从马铃薯健康植株中分离得到一株内生真菌(E-19),根据形态学特征和18S r DNA序列分析,将其鉴定为Bipolaris eleusines。平板对峙培养结果显示,该菌对马铃薯晚疫病菌有较强的抑制作用;采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备HPLC等方法从Bipolaris eleusines发酵液中分离纯化获得6个化合物;通过波谱学数据及理化性质确定它们的结构分别为:Isosativenediol(1)、Dihydroprehelminthosporo(2)、Helminthosporol(3)、Drechslerines C(4)、Drechslerines A(5)、Secolongifolene diol(6)。以上化合物均为首次从该菌中分离得到。This paper reported the isolation and identification of chemical components of the fermentation products of endophytic fungi E-19,isolated from Solanum tuberosum. This fungus was identified as Bipolaris eleusines on the basis of phylogenetic tree analysis of its 18S rDNA sequence. The secondary metabolites were isolated and purified using gel col- umn chromatography, semi-preparative HPLC and Sephadex LH-20, Their structures were determined to be isosativenediol （1）, dihydroprehelminthosporo （2）, helminthosporol （3）, drechslerines C （4）, drechslerines A （5）, secolongifolene diol （6） on basis of spectroscopic analysis and comparison with literatures.

关 键 词：马铃薯 内生真菌 BIPOLARIS eleusines 化学成分 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]