响应面法优化金刷把多糖提取工艺  

Optimization of Extraction of Polysaccharide from Lethariella cladonioides Using Response Surface Methodology

作　　者：刘婷[1] 尚姣 王慧[1] 郑娟娟[1] 赵洁[1] 房敏峰[1] 

机构地区：[1]西部资源生物与现代生物技术教育部重点实验室西北大学生命科学学院,西安710069

出　　处：《天然产物研究与开发》2015年第10期1759-1764,共6页Natural Product Research and Development

基　　金：国家自然科学基金(J1210063);陕西省食品药品监督管理局药材标准起草项目(2009-11);陕西省大学生创新创业训练计划(2014100697116)

摘　　要：利用响应面法优化金刷把多糖的提取工艺。在单因素试验的基础上,选择提取温度、提取时间、料液比为自变量,以多糖提取率为响应值,进行Box-Benhnken中心组合实验设计,应用响应曲面分析方法优化提取条件,得到金刷把多糖最佳提取工艺条件如下:提取温度95℃,提取时间2.5 h,料液比1∶20 g/m L,此时金刷把多糖提取率的理论预测值为4.62%,最优条件下多糖得率的实验值为4.42%,与理论值的相对误差为4.3%。经过响应面法优化提取工艺,提高了提取率,适用于金刷把粗多糖的提取。The objective of this study was to optimize the extraction of polysaccharide from Lethariella cladonioides （NyL.） Krog through response surface methodology. Based on sing!e factor experiments, extraction temperature, extraction duration, solid-liquid ratio were selected for Box-Behnken central composite design. By means of regression analysis, the results showed that the optimal conditions to extract polysaccharide from L. cladonioides were as follows：extraction temperature of 95 ℃ ,extraction duration of 2.5 h, solid-liquid ratio of 20 mL/g. Under these conditions,the extraction yield of polysaccharide reached 4.42%. Compared with the theoretical value （4.62%） ,the relative error was 4.3%. The results showed that the optimized process improved extraction efficiency. It can be used in practical forecasting.

关 键 词：金刷把 地衣多糖 响应面法 

分 类 号：TS201[轻工技术与工程—食品科学]