氧化苦参碱对子宫内膜癌细胞侵袭转移的抑制作用及其机制探讨  

Effects and mechanisms of oxymatrine on invasion and metastasis of endometrial carcinoma cells

作　　者：吴晓玲[1] 蔡东阁[1] 刘变利[1] 孙师元[1] 李牧[1] 

机构地区：[1]西安交通大学第二附属医院,西安710004

出　　处：《陕西医学杂志》2015年第10期1284-1285,1289,共3页Shaanxi Medical Journal

基　　金：陕西省科技攻关计划项目(2014K11-01-01-18)

摘　　要：目的:探讨氧化苦参碱对人子宫内膜癌细胞HEC-1-B增殖、侵袭转移的作用及其机制。方法:MTT法检测细胞增殖抑制率;Transwell小室法观察细胞侵袭转移的改变;Western blot及Real-time PCR法检测p38、p-p38、MMP-2和MMP-9表达水平的变化。结果:一定浓度氧化苦参碱可显著抑制子宫内膜癌细胞HEC-1-B的增殖、侵袭转移,并呈时间和剂量依赖性。氧化苦参碱可显著降低HEC-1-B细胞中p38及MMPs的表达水平。联合应用SB203580能够有效增强氧化苦参碱的抗肿瘤细胞侵袭转移能力。结论:氧化苦参碱可以抑制子宫内膜癌细胞的增殖、侵袭转移,并下调p38磷酸化以及MMP2、MMP9的表达。Objective：To investigate the effects and mechanisms of oxymatrine on invasion and metastasis of Endometrial carcinoma HEC‐1‐B cells .Methods ：HEC‐1‐B cells were cultured in vitro and treated by 0 ,0 .5 ,1 .0 and 1 .5 mg/ml oxymatrine .Then cell growth curves assay and cell invasion assay using Transwell chamber was used to evaluate the effects of oxymatrine on invasion of HEC‐1‐B cells .The expression of p38 ,p‐p38 ,matrix metal‐loproteinases‐2（MMP‐2） and MMP‐9 were analysed by Western blot and Real‐time PCR .Results ：Compared with control group ,oxymatrine （0 .5 ,1 .0 and 1 .5 mg/ml） treated groups significantly inhibited the proliferation and inva‐sive ability of HEC‐1‐B cells in a concentration‐and time‐dependent manner .In addition ,oxymatrine reduced the phosphorylation level of p38 protein and the mRNA levels of MMP‐2 and MMP‐9 .Moreover ,a p38 inhibitor‐SB203580 and oxymatrine excert synergistic inhibition effects on the expression of MMP‐2 and MMP‐9 in HEC‐1‐B cells .Conclusion：These findings suggest that a potential mechanism by which oxymatrine has an anti‐metastatic effects on endometrial cancer cells via directly down‐regulating the p38 signaling pathway .

关 键 词：子宫内膜肿瘤 氧化苦参碱 肿瘤转移 基因表达调控  肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]