^(60)Co-γ射线辐照对苦丁茶冬青成叶样液抗氧化活性与颜色的影响  

Effect of ^(60)Co-γ Ray Irradiation on the Antioxidant Activities and the Colors of the Liquid Samples of Extracted Mature Leaves in Ilex Kudingcha C. J. Tseng

作　　者：郭燕[1] 赵峰[2] 刘国民[1] 罗志平 柳贤德[4] 李丽辉 

机构地区：[1]海南大学苦丁茶研究所,海南海口570228 [2]海南广播电视大学,海南海口570208 [3]湖南省核农学与航天育种研究所,湖南长沙421125 [4]海南大学农学院,海南海口570208

出　　处：《热带生物学报》2015年第3期273-278,292,共7页Journal of Tropical Biology

基　　金：国家自然科学基金项目(39860048);海南省自然科学基金项目(312099)

摘　　要：分别用0,10,20,30 kGy剂量的60Co-γ射线对苦丁茶冬青成叶的15℃水提物、90℃热水提取物、70%甲醇提取物、70%乙醇提取物及70%丙酮提取物的样液进行辐照,采用ABTS和DPPH法测定样液在辐照前后的抗氧化活性,并观察、比较样液在辐照前后的颜色变化。结果表明:苦丁茶冬青成叶的15℃水提物样液经过60Co-γ射线辐照后,抗氧化活性有小幅提升;而90℃热水、70%甲醇、70%乙醇和70%丙酮的提取物样液的抗氧化活性则在辐照前后无明显差异。不同溶剂的苦丁茶冬青提取物样液在用不同剂量的60Co-γ射线辐照后,其颜色在一定程度上均有所褪减。其中,经20 kGy照射后的70%乙醇提取物的颜色最浅,10kGy照射后的甲醇提取物颜色次之。苦丁茶冬青成叶的提取物作为抗氧化剂应用于肉食品保鲜防腐、保健品或化妆品生产时,从食品和化妆品安全、抗氧化活性强弱以及颜色深浅等方面综合考虑,宜选用甲醇提取,辐照剂量以10 kGy较合适。The liquid samples of water-extract（ 15 ℃）,hot water extract（ 90 ℃）,methanol extract（ 70%）,alcohol extract（ 70%） and acetone extract（ 70%） from the mature leaves of Ilex kudingcha C. J. Tseng were irradiated with60Co-γ ray at respective doses of 0（ CK）,10,20 and 30 k Gy. The antioxidant activities of the liquid samples were determined via ABTS and DPPH methods and the colors of the liquid samples before and after irradiation were observed and compared. The results showed that the antioxidant activity of the liquid sample of the water extract（ room temperature） was slightly increased after irradiation of60Co-γ ray. There was,however,no significant difference in the liquid samples extracted with hot water（ 90 ℃）,70% methanol,70% alcohol and 70% acetone. The colors of all the liquid samples extracted with 5 different solvents from the mature leaves in I. kudingcha faded in some degrees. of which the color of the liquid sample extracted with 70% alcohol was the palest,followed by that of the liquid sample extracted with 70% methanol. When the extracts are used for food fresh-keeping and for food or cosmetics preparation,it is recommended to use the extract with 70%methanol as solvent and treat with60Co-γ ray at the dose of 10 k Gy to ensure the security of food and cosmetics.

关 键 词：苦丁茶冬青 60Co-γ射线辐照 抗氧化活性 颜色 

分 类 号：Q949.754.6[生物学—植物学]