芬太尼对食管鳞癌细胞株Eca109侵袭和基质金属蛋白酶表达的影响  

Effect of fentanyl on invasion of Eca109 cells and MMP expression in esophageal squamous cell carcinoma

作　　者：张明鑫[1] 何平[2] 张灵敏[2] 王景杰[1] 

机构地区：[1]第四军医大学唐都医院消化内科,西安710038 [2]西安交通大学第一附属医院麻醉科

出　　处：《山西医科大学学报》2015年第10期961-964,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81302055)

摘　　要：目的探讨不同浓度芬太尼对食管鳞癌细胞株Eca109侵袭能力的影响及可能机制。方法选用不同浓度芬太尼(0.5,5,50,500 ng/ml)干预食管鳞癌细胞株Eca109,以常规培养的Eca109作为对照,采用Transwell检测细胞侵袭能力,并应用RT-PCR和Western blot探讨芬太尼对MMP-2和MMP-9的mRNA和蛋白表达的影响。结果各浓度组芬太尼孵育Eca109细胞48 h,与对照组相比,均能显著抑制细胞的侵袭(P<0.05),且抑制率随着浓度升高而升高,呈浓度依赖性。芬太尼孵育Eca109细胞48 h,与对照组相比,各浓度组均能抑制MMP-2和MMP-9的mRNA和蛋白的表达,同样呈浓度依赖性(P<0.05)。结论芬太尼可抑制食管鳞癌细胞株Eca109的侵袭,这种作用可能是基于对MMP-2和MMP-9表达的抑制。Objective To investigate the effects of fentanyl on the invasion of esophageal squamous cell carcinoma cell line Eca109 and its possible mechanism. Methods Eca109 cells were treated with different concentrations of fentanyl（ 0. 5,5,50,500 ng / ml）,respectively,and the cells cultured with conventional medium were chosen as controls. Transwell assay was applied to test the cell invasion. RT-PCR and Western blot were used to detect the expression of matrix metalloproteinase 2（ MMP-2） and matrix metalloproteinase 9（ MMP-9） at mRNA and protein levels respectively. Results Transwell assay revealed that the fentanyl inhibited cell invasion at 48 h in a dose dependent manner（ P〈 0. 05）. RT-PCR and Western blot results showed that the fentanyl inhibited the mRNA and protein levels of MMP-2 and MMP-9 in a dose department manner（ P〈 0. 05）. Conclusion Fentanyl could inhibit the cell invasion of ESCC cell line Eca109 probably through down-regulating the expression of MMP-2 and MMP-9.

关 键 词：芬太尼 食管鳞癌 细胞侵袭 基质金属蛋白酶 

分 类 号：R735.1[医药卫生—肿瘤]