高蛋氨酸饮食对大鼠血管平滑肌细胞作用的实验研究  

Experimental Study Of High Methionine Diet On Vascular Smooth Muscle Cells

作　　者：陈立杰[1] 常明秀[2] 靳京美[3] 曹月[1] 王锐[1] 张雪梅[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经内科,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第四医院神经内科 [3]大庆龙南医院内科

出　　处：《中国实验诊断学》2015年第10期1622-1625,共4页Chinese Journal of Laboratory Diagnosis

基　　金：黑龙江省教育厅科学技术研究资助项目(12511266)

摘　　要：目的研究高蛋氨酸(Methionine,Met)饮食对大鼠血浆同型半胱氨酸(Homocysteine,Hcy)和硫腺苷同型半胱氨酸(S-adenosylhomocysteine,SAH)浓度及颈动脉血管平滑肌细胞的影响,同时观察叶酸、维生素B12对其血管损害的保护作用。方法选取Wistar雄性大鼠21只,随机分为三组:对照组给予正常饲料,高蛋氨酸组给予添加1%高蛋氨酸的饲料;干扰组给予1%高蛋氨酸同时添加叶酸、维生素B12饲料。饲喂动物10周后留取腹主动脉血检测Hcy、SAH浓度,留取颈总动脉标本行HE染色,并计算平滑肌厚度指数(Smooth muscle thickness index,SMTI)。结果高蛋氨酸组大鼠血浆Hcy水平较对照组及干扰组明显增高(P<0.05),血浆SAH水平较对照组明显下降(P<0.05),SMTI较对照组及干扰组明显增高(P<0.05)。结论高蛋氨酸饮食对Wistar雄性大鼠具有毒性作用,能引起血浆Hcy水平升高,SAH水平下降,引起动脉平滑肌损伤,并且这种血管损伤可能是由于血浆Hcy水平增高所致。叶酸、维生素B12具有拮抗高蛋氨酸饮食所致的高Hcy血症,对血管损伤具有一定的保护作用。Objective To investigate the impact of High methionine diet on the concentration of Homocysteine（Hcy）and S-adenosylhomocysteine（SAH）and the carotid artery vascular smooth muscle cells in rats,observe the protective effect of folic acid and vitamin B12 on the vascular damage.Methods 21 male Wistar rats were selected,randomly divided into three groups：The control group received normal diet,the high methionine group were added 1%high methionine on the basis of normal diet,the intervention group adding folic acid and vitamin B12 on the basis of the high methionine group.Feeding these diets for 10 weeks,then collecting the abdominal aortic blood for detection the concentrations of Hcy and SAH,isolating the carotid artery for HE staining and calculating the smooth muscle thickness index（SMTI）.Results The concentration of homocysteine in the high methionine group was significantly higher than the control group and the intervention group（P〈0.05）,the SAH level was significantly reduced contrary to the control group（P〈0.05）,SMTI was significantly higher than the control group and the intervention group（P〈0.05）.Conclusion High methionine diet has a toxic effect on the male Wistar rats,it can lead to the elevated of Hcy concentration,the decreased of plasma SAH level and the damaged of vascular smooth muscle cell,besides,the elevated homocysteine level may be the reason that causes this vascular damage.Folic acid,vitamin B12 have the effects that antagonist the hyperhomocystinemia which are leaded by the high methionine diet and has a protective effect on the vascular damage.

关 键 词：蛋氨酸 同型半胱氨酸 硫腺苷同型半胱氨酸 血管平滑肌细胞 

分 类 号：R743[医药卫生—神经病学与精神病学]