DC、CIK联合培养对肿瘤细胞杀伤作用的实验研究  

Experiment study of killing effect of DC and CIK co-cultured on tumor cells

作　　者：赵鑫[1] 常颖[1] 刘玉侠[2] 卢卫平[1] 王宝[1] 王哲[1] 于鸿[2] 王启文[1] 王斌[1] 

机构地区：[1]吉林省肿瘤医院,吉林长春130012 [2]吉林省肿瘤防治研究所

出　　处：《中国实验诊断学》2015年第10期1636-1638,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨DC(dendritic cells)、CIK(cytokine-induced killer cells)联合培养对肺腺癌细胞A549的杀伤活性的影响,并与单独CIK对A549杀伤活性进行比较,为肿瘤生物治疗提供有效方法。方法分别培养DC、CIK细胞,经鉴定后进行联合培养,联合培养3天后,对A549细胞进行杀伤活性实验,检测DC联合CIK及单独CIK对A549的杀伤活性,同时用流式细胞检测DC、CIK联合培养细胞及单独CIK细胞中淋巴细胞亚群的表达。结果 DC联合CIK对A549的杀伤活性为88.38%,CIK对A549的杀伤活性为66.24%,两者对A549的杀伤活性比较有显著差异(P<0.01);DC、CIK联合培养的淋巴细胞亚群中的CD3+、CD4+、NK、NKT、CD3+HLA-DR+、CD8+CD28+的数量均高于CIK细胞。结论 DC、CIK联合培养对肺癌细胞A549的杀伤活性明显高于单独CIK对A549的杀伤活性(P<0.01);抗肿瘤淋巴细胞(CD3+、CD4+、NK、NKT、CD3+HLA-DR+、CD8+CD28+)数量也明显高于CIK细胞。Objective To discussion of cytotoxicity effects of DC（dendritic cells）and CIK（cytokine-induced cells）co-cultured on lung adenocarcinoma cell line A549,and Compare with CIK alone on A549 killing activity,Provide an effective method for tumor biotherapy.Methods respectively cultured DC,CIK cell,and co-cultured after the identification,after 3days,to killing activity experiments on A549 cell,detect cytotoxicity of DC joint CIK and separate CIK against A549,While using flow cytometry detect lymphocyte subsets expression of DC joint CIK and CIK alone.Results The cytotoxicity of DC joint CIK on A549 was 88.38%,CIK was 66.24%,there were significant differences of cytotoxic activity on A549（P〈0.01）between,the number of lymphocyte subsets（CD3＋,CD4＋,NK,NKT,CD3＋HLADR＋,CD8＋CD28＋cell）of DC joint CIK was significantly higher than CIK.Conclusion The killing activity of DC and CIK co-cultured on A549 lung cancer has significantly higher than that of single CIK cytotoxicity on A549（P〈0.01）.

关 键 词：树突状细胞 CIK细胞 T淋巴细胞亚群 杀伤活性 

分 类 号：R734.2[医药卫生—肿瘤]