茶叶生产废料中表没食子儿茶素没食子酸酯的纯化工艺  

Purification Process of Epigallocatechin-3-gallate in Tea Production Waste

作　　者：许敏[1] 黄思勇[1] 周光龙[1] 胡平[1] 彭经寿[1] 金显平[1] 

机构地区：[1]恩施职业技术学院,湖北恩施445000

出　　处：《湖北农业科学》2015年第18期4570-4572,4577,共4页Hubei Agricultural Sciences

基　　金：恩施州级科技计划项目(2008A29)

摘　　要：分析了茶叶生产废料中表没食子儿茶素没食子酸酯(EGCG)的纯化工艺,并采用大孔树脂对其进行分离纯化。结果表明,运用静态吸附与动态吸附相结合的方法分析得到,适用于茶叶生产废料中EGCG分离纯化用的大孔吸附树脂为D101。其最佳分离纯化条件为:将20.0 mg/m L上柱液以1.5 BV/h上柱4.5 BV,使用20%乙醇以0.5 BV/h洗脱4.0 BV。An orthogonal test was used to optimize the extraction technology of EGCG from tea production waste,and to separate and purify the Epigallocatechin-3-gallate by macroporous resin.The results showed the optimal conditions for separation and purification were： D101 macroporous resin, the concentration of sample was 20.0 mg/m L, the volume of sample was 4.5times of column volume, flow rate was 1.5 BV / h 20% ethanol for elution, elution velocity was 0.5 BV / h while elution volume was 4.0 BV.

关 键 词：茶叶生产废料 表没食子儿茶素没食子酸酯(EGCG) 纯化 

分 类 号：S571.1[农业科学—茶叶生产加工] TS272.2[农业科学—作物学]