云南切梢小蠹气味结合蛋白的同源模建  

Homology modeling of the odorant binding protein of the pine shoot beetle, Tomicus yunnanensis

作　　者：汪宇[1] 孙浩[2] 朱家颖[1] 赵宁[1] 杨斌[1] 

机构地区：[1]西南林业大学云南省森林灾害预警与控制重点实验室,昆明650224 [2]西南林业大学理学院,昆明650224

出　　处：《应用昆虫学报》2015年第5期1223-1228,共6页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金项目(31260179);国家林业局林业公益行业科研专项(201004067)

摘　　要：【目的】为了研究云南切梢小蠹Tomicus yunnanensis气味结合蛋白(Odorant binding proteins,OBPs)参与其嗅觉识别过程的机理奠定基础,本文对云南切梢小蠹OBP进行了同源模建及评价分析。【方法】采用同源模建方法构建了云南切梢小蠹OBP的三维结构,并利用Procheck、Verify_3D和ERRAT等方法评估了构建模型的可靠性。【结果】对优化后的模建蛋白的结果分析发现,序列中有6个保守的半胱氨酸位点,具有气味结合蛋白的典型特征,并且Procheck、Verify_3D和ERRAT对模型的评价分数较高,结构具有很高的可能性。【结论】基于已知的同源蛋白结构信息和模建蛋白的一级序列信息,预测序列已知但结构未知的蛋白空间结构,为今后研究该云南切梢小蠹气味结合蛋白的功能结构域奠定基础。[Objectives] This study aims to provide the basis for revealing the mechanism of the odorant binding protein of the pine shoot beetle, Tomicus yunnanensis involved in olfactory reorganization [Methods] The three dimensional structure of T. yunnanensis＇ s OBP was constructed using the homology modeling method and the reliability of the model was evaluated by Procheck, Verify_3D and ERRATERRAT. [Results] Analysis of the optimized structure found that the OBP of T. yunnanensis contains 6 conservative cysteine residues in its sequence. Evaluation of the model by Procheck, Verify_3D and ERRATERRAT indicated that it is reasonable and reliable. [Conclusion] The results showed that it is possible to predict the structure of a protein that has a known sequence with unknown spatial structure based on known structural information of its homologous proteins. The results provide a foundation for further research on the functional structure domain of T. yunnanensis OBP.

关 键 词：气味结合蛋白 同源模建 三维结构 云南切梢小蠹 

分 类 号：S763.38[农业科学—森林保护学]