组蛋白H2B单泛素化在原发性结肠腺癌中的表达及意义  

Clinical implication of histone H2B monoubiquitination level in primary colonic carcinoma

作　　者：杨群[1] 刘聪[2] 王思[2] 

机构地区：[1]四川省医学科学院.四川省人民医院病理科,成都610072 [2]四川大学华西第二医院妇产科,成都610041

出　　处：《临床与实验病理学杂志》2015年第10期1095-1098,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨组蛋白H2B单泛素化(histone H2B monoubiquitination,u H2B)在原发性结肠腺癌中的表达及其与临床病理参数的关系。方法采用免疫组化ABC法检测116例原发性结肠腺癌及15例正常结肠黏膜组织中u H2B的表达,并复习相关文献。结果 u H2B在低分化原发性结肠腺癌中的阳性率(19.4%,6/31)显著低于高+中分化腺癌(49.4%,42/85)及正常结肠黏膜组织(86.7%,13/15),差异有统计学意义(P<0.05)。u H2B与肿瘤分化程度、Dukes分期、TNM分期及淋巴结转移相关(P<0.05),与患者性别、年龄无关(P>0.05)。结论 u H2B作为一种抑癌因子可能与原发性结肠腺癌的恶化过程密切相关。其可能会成为原发性结肠腺癌早期检测、治疗及预后评估的新分子生物学标志并应用于临床,而其在结肠腺癌中的具体作用机制仍有待进一步研究。Purpose To explore the role of histone H2B monoubiquitination in primary colonic carcinoma and clinicopathological pa-rameters of colonic carcinoma. Methods To detecte the expression of histone H2B monoubiquitination in 116 cases of primary colonic carcinoma and 15 cases of normal colonic mucosal tissue by avidin-biotin-peroxidase complex （ ABC）-immunohistochemistry. Results The level of histone H2B monoubiquitination was significantly reduced in poorly differentiated colonic carcinoma 19. 4% （6/31） compared with that of well-differentiated colonic carcinoma 49. 4%（42/85）, moderately differentiated colonic carcinoma 49. 4% （42/85） and normal colonic mucosal tissue 86. 7% （13/15） （P〈0. 05）. The expression of histone H2B monoubiquitination was closely correlated with tumor differentiation, Dukes stage, TNM stage and lymph node metastasis （P〈0. 05）, but not depended on sex or age （P〉0. 05）. Conclusion Histone H2B monoubiquitination is obviously associated with the progression of primary colonic carcinoma. Although its specific mechanism still remains unclear, histone H2B monoubiquitination could be a novel potential molecular marker for early diagnosis, clinic treatment and prognosis evaluation.

关 键 词：结肠肿瘤 组蛋白H2 B单泛素化 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤]