母子间口腔群落结构的相关性分析  

Similarity analysis of the oral bacteria community by polymerase chain reaction-denaturing gradient gel electrophoresis

作　　者：于淼[1] 仪虹[2] 郭笑 

机构地区：[1]潍坊市人民医院口腔科,潍坊261041 [2]哈尔滨医科大学附属口腔医院牙体牙髓病科,哈尔滨150001 [3]大庆市第五医院口腔科,大庆163000

出　　处：《国际口腔医学杂志》2015年第6期664-667,共4页International Journal of Stomatology

摘　　要：目的应用聚合酶链反应（PCR）．变性梯度凝胶电泳（DGGE）技术，分析30对母子口腔唾液中菌群的相似性。方法选取3～5岁高龋、中龋和无龋儿童及其母亲各10对作为研究对象，取其非刺激性全唾液，分别提取细菌总DNA，进行16SrRNA的PCR扩增及DGGE分析。结果母子间口腔菌群组成具高度相似性。其中，无龋组母子间配对相似系数明显低于高龋组及中龋组（P〈0．05）；高龋儿童的母亲平均龋失补牙面数（DMFS）明显高于中龋和无龋儿童的母亲（P〈0．05）。结论母亲是儿童口腔菌群获得的主要来源。Objective This study aimed to analyze the similarity of the oral bacteria community in 30 mother-child dyads via polymerase chain reaction（PCR）-based denaturing gradient gel electrophoresis. Methods Unstimulated saliva samples were obtained from 30 mother-child pairs： 10 in caries-susceptive group, l0 in caries-moderate group, and l0 in caries-free group. DNAs were extracted for PCR amplification and denaturing gradient gel electrophoresis analysis. Results The compositions of the oral bacteria community in mother and child have a high degree of similarity. The oral bacteria community of children matched that of their mothers. The dice similarity coefficient of mother-child in the caries- free group was more statistically significant compared with the two other groups（P〈0.05）. However, mothers of caries- susceptive children had significantly higher decay missing filling surface（DMFS） scores than mothers of caries-free and caries-moderate children（P〈0.05）. Conclusion Children obtained oral bacteria from their mothers.

关 键 词：唾液 口腔 细菌群落 变性梯度凝胶电泳 

分 类 号：R781.1[医药卫生—口腔医学]