Construction of a genetic conditional learning system in Escherichia coli  

作　　者：CHEN Mei XU Jin 

机构地区：[1]School of Information Engineering, Minzu University of China [2]Institute of Software, School of Electronics Engineering and Computer Science,Key Laboratory for High Confidence Software Technologies, Ministry of Education, Peking University

出　　处：《Science China(Information Sciences)》2015年第11期172-177,共6页中国科学（信息科学）（英文版）

基　　金：supported by National Basic Research Program of China(973 Program)(Grant Nos.2013CB3296-01,2013CB329602);National Natural Science Foundation of China(Grant Nos.61127005,60910002,61379059,61472333,61402382,30970960);"Top University,Top Discipline"Funds for Minzu University of China(Grant No.10301-0150200518)

摘　　要：Currently, there are a lot of man-made biomolecular computing systems. However, it is rare to see man-made biomolecular intelligence system. Learning ability is one of the basic abilities of intelligence. Many organisms have learning ability naturally. In this paper, we have successfully designed, constructed, and tested a genetic conditional learning system in the bacterium Escherichia coli. The system can recognize ‘bad guy＇ signal with the help of ‘study＇ signal. The function was realized by combining biomolecular AND gate and memory inverter. The AND gate used riboswitch as computation tool, and the memory inverter used Cre recombinase.Flow cytometry was used to verify the function of the system. This study is an attempt of constructing manmade biomolecular intelligence system. The system may lead to new applications for biomolecular computing,artificial intelligence, and biotherapy.Currently, there are a lot of man-made biomolecular computing systems. However, it is rare to see man-made biomolecular intelligence system. Learning ability is one of the basic abilities of intelligence. Many organisms have learning ability naturally. In this paper, we have successfully designed, constructed, and tested a genetic conditional learning system in the bacterium Escherichia coli. The system can recognize ‘bad guy＇ signal with the help of ‘study＇ signal. The function was realized by combining biomolecular AND gate and memory inverter. The AND gate used riboswitch as computation tool, and the memory inverter used Cre recombinase.Flow cytometry was used to verify the function of the system. This study is an attempt of constructing manmade biomolecular intelligence system. The system may lead to new applications for biomolecular computing,artificial intelligence, and biotherapy.

关 键 词：biomolecular computing artificial intelligence conditional learning MEMORY RIBOSWITCH DNA recombinase 

分 类 号：Q93[生物学—微生物学] TP18[自动化与计算机技术—控制理论与控制工程]