siRNA干扰即刻早期基因Egr-1对汉防己甲素诱导的胰腺癌细胞自噬、凋亡的作用研究  

Effect of RNA Interference Targeting Egr-1 Inhibite Tetrandrine-Induced Autophagy and Apoptosis of Pancreatic PaTu8988 Cells in vitro

作　　者：彭琬昕[1] 万燕雅 葛璐[1] 金洁[1] 龚爱华[1] 吴朝阳[2] 

机构地区：[1]江苏大学医学院生物系,江苏镇江212013 [2]江苏大学附属人民医院肿瘤放疗科,江苏镇江212013

出　　处：《南京师大学报（自然科学版）》2015年第3期71-75,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31100964);江苏省博士后基金(1402102C);江苏大学高级人才科研启动基金(10JDG045)

摘　　要：本实验将胰腺癌细胞Pa Tu8988分别转染无关干扰对照组(si RNA-Scramble)和干扰即刻早期基因Egr-1组(si RNA-Egr-1),再用不同浓度的汉防己甲素处理24 h后,CCK-8法检测不同浓度的汉防己甲素对细胞活力的影响;Western blot检测细胞自噬和凋亡相关蛋白表达水平的改变.结果表明:(1)低浓度的汉防己甲素能显著激活Pa Tu8988细胞自噬,但对增殖和凋亡无明显影响;(2)与对照组相比,转染了si RNA-Egr-1组自噬相关蛋白Be-clin-1表达量显著下降,LC3II/LC3I比值下调;凋亡相关蛋白Bcl2/Bax的比值降低.上述结果表明,干扰即刻早期基因Egr-1的表达可以有效抑制低浓度汉防己甲素引起的胰腺癌细胞Pa Tu8988自噬,促进细胞凋亡.Human pancreatic cancer cells Pa Tu8988 were transfected with si RNA-Scramble and si RNA-Egr-1 for 24 hrespectively,then treated with tetrandrine for another 24 h. Treated by different concentration of tetrandrine,the cytotox-ic effect of tetrandrine was examined by CCK-8,and expression of autophagy-related protein were detected by Westernblot. The expression of cell autophagy- and apoptosis-related protein were detected by Western blot. All of the results in-dicated that comparing with control group,siEgr-1 significantly attenuates tetrandrine-induced autophagy and promotesthe apoptosis of Pa Tu8988 cells.

关 键 词：胰腺癌 汉防己甲素 EGR-1 细胞自噬 细胞凋亡 

分 类 号：R730.5[医药卫生—肿瘤]