骨髓间充质干细胞联合青霉胺治疗肝豆状核变性肝纤维化的效果  

Advances in research of the reversal of hepatolenticular degeneration with liver fibrosis by transplantation of bone marrow mesenchymal stem cells

作　　者：张东锋[1] 王煜姝[1] 腾军放[2] 

机构地区：[1]郑州人民医院神经内科帕金森肝豆状核变性病区,450000 [2]郑州大学第一附属医院神经内科,450000

出　　处：《实用医学杂志》2015年第19期3195-3199,共5页The Journal of Practical Medicine

基　　金：郑州市科技计划项目(编号:121PPTGG494-1)

摘　　要：目的:探讨骨髓间充质干细胞(BMSCs)对肝豆状核变性肝纤维化患者的治疗作用。方法 :60例确诊肝豆状核变性肝纤维化患者,随机分为3组:青霉胺组、BMSCs组和BMSCs+青霉胺组,口服青霉胺剂量为每日40 mg/kg,将采集的BMSCs液2 m L+生理盐水100 m L经静脉输注入人体。分别于治疗前后做肝组织病理活检,检测0、4、8、12周血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、层黏蛋白(LN)、Ⅳ型胶原(CⅣ)、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶抑制剂-1(TIMP-1)的变化。结果:BMSCs+青霉胺组肝组织活检肝纤维化明显改善;各组血清中HA、PCⅢ、LN、CⅣ、TIMP-1、MMP-1均持续降低,BMSCs+青霉胺组血清中各细胞因子的改善比其他组明显,差异具有统计学意义(P<0.05)。结论:BMSCs联合青霉胺治疗肝豆状核变性合并肝纤维化有明显改善作用。Objective To evaluate the effect of bone marrow stem ceils （BMSCs） transplantation in the treatment of hepatolenticular degeneration of liver fibrosis. Methods Sixty cases with confirmed hepatolenticular degeneration of liver fibrosis were randomly divided into 3 groups： penicillamine group [40 mg/（kg-d）], BMSCs group and BMSCs ＋ penicillamine group. Autologous BMSCs （2 mL） were injected into vein with normal saline （100 mL）. Liver tissue pathology biopsy was inspected and the changes in HA, PC llI, LN, CIV, TIMP- 1 and MMP-1 were observed at 0, 4th, 8th and 12th week during the therapeutic process. Results The level of serum fibrotic markers were reduced in every group, while the changes in BMSCs ＋ penicillamine group were especially obvious （P 〈 0.05）. Conclusion Penicillamine combined with BMSCs was effective in the therapy of hepatolenticular degeneration of liver fibrosis.

关 键 词：肝豆状核变性 肝纤维化 骨髓间充质干细胞 青霉胺 

分 类 号：R575.24[医药卫生—消化系统]