羟丁酸钠对缺血-再灌注损伤小鼠小肠功能的影响  

Effect of sodium oxybate on intestinal function in mice with intestinal ischemia-reperfusion injury

作　　者：黄潇[1] 齐家磊 韩艳艳[1] 周宇[1] 蒋涛[1] 张励才[2] 

机构地区：[1]徐州医学院麻醉学院,江苏省221004 [2]江苏省麻醉学重点实验室

出　　处：《江苏医药》2015年第20期2370-2372,共3页Jiangsu Medical Journal

基　　金：江苏省高等学校大学生创新创业训练计划(201310313012Z)

摘　　要：目的探讨羟丁酸钠对缺血-再灌注(I-R)损伤小鼠小肠功能的影响。方法将90只昆明种小鼠随机均分为三组:S组为假手术对照;其余两组采用肠系膜上动脉夹闭20min、再灌注1h建立肠I-R损伤模型。I-R组为模型对照,I-R+SO组于肠I-R损伤前30min腹腔注射羟丁酸钠50mg/kg预处理。术后用亚甲蓝灌胃法检测小肠蠕动功能,木糖灌胃法检测小肠吸收功能,ELISA法检测小肠黏膜IL-6含量。结果与I-R组相比,I-R+SO组亚甲蓝移动率升高(P<0.05),木糖吸收量和小肠黏膜中IL-6的含量降低(P<0.05)。结论羟丁酸钠对小鼠小肠I-R损伤有保护作用,且可下调IL-6的表达。Objective To investigate the effect of sodium oxybate（SO）on intestinal function in mice with intestinal ischemia-reperfusion（I-R）injury.Methods Ninety Kunming mice were equally and randomly divided into 3groups.Group S was taken as sham operation control.The I-R injury models of mice were established by superior mesenteric artery occlusion for 20 minutes and reperfusion for an hour in groups of I-R（model control）and I-R＋SO（intraperitoneally injected SO 50mg/kg at30 minutes before intestinal I-R injury）.The propelling rate of methylene blue and xylose absorption dose were used to evaluate the functions of intestinal peristalsis and absorption.The IL-6expression in small intestine after operation was detected by ELISA.Results Compared with group I-R,the propelling rate of methylene blue was increased,and xylose absorption dose and IL-6expression in small intestine were decreased in group I-R＋SO（P〈0.05）.Conclusion SO has a protective effect on small intestine with I-R injury and can down-regulate the expression of IL-6in small intestine of mice.

关 键 词：羟丁酸钠 缺血-再灌注损伤 白细胞介素6 

分 类 号：R743[医药卫生—神经病学与精神病学]